Flow cytometric analysis of Human CD99 expression on human peripheral blood. Human peripheral blood was stained with FITC Mouse IgG2a, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD99 Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Human CD99 Antibody (S-R646)
FITC Mouse Anti-Human CD99 Antibody (S-R646)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD99 |
| Synonyms | CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; MIC2; MIC2X; MIC2Y; |
| Location | Membrane |
| Accession | P14209 |
| Clone Number | S-R646 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD99, also known as MIC2, is a 32 kDa transmembrane glycoprotein encoded by the CD99 gene, located in the pseudoautosomal region of human chromosomes X and Y. It plays crucial roles in various biological processes, including cell adhesion, migration, apoptosis, and immune responses. CD99 is highly expressed in hematopoietic cells, thymus, endothelial cells, and certain cancers. In normal tissues, it is expressed at low levels but shows differential expression in bone marrow and lymphoid cells.
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