WB result of ERAP1 Recombinant Rabbit mAb
Primary antibody: ERAP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled K562 whole cell lysate 20 µg
Lane 2: unboiled A431 whole cell lysate 20 µg
Lane 3: unboiled HDLM-2 whole cell lysate 20 µg
Lane 4: unboiled HUVEC whole cell lysate 20 µg
Lane 5: unboiled Jurkat whole cell lysate 20 µg
Lane 6: unboiled HepG2 whole cell lysate 20 µg
Lane 7: unboiled Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
ERAP1 Recombinant Rabbit mAb (S-2509-38)
ERAP1 Recombinant Rabbit mAb (S-2509-38)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ERAP1 |
| Synonyms | Endoplasmic reticulum aminopeptidase 1; ARTS-1; Adipocyte-derived leucine aminopeptidase (A-LAP); Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase (PILS-AP); Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; APPILS; ARTS1; KIAA0525 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q9NZ08 |
| Clone Number | S-2509-38 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, A431, HDLM-2, HUVEC, Jurkat, HepG2, Ramos, mouse liver, mouse heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
ERAP1 (Endoplasmic Reticulum Aminopeptidase 1) is a zinc-dependent metalloaminopeptidase localized in the endoplasmic reticulum that plays a critical role in antigen processing by trimming N-terminal residues of peptides to optimize their length (8–10 amino acids) for loading onto MHC class I molecules, thereby shaping the immunopeptidome and influencing immune responses; it also modulates inflammation by cleaving cytokine receptors and is genetically associated with autoimmune diseases such as ankylosing spondylitis and Behçet’s disease, particularly in individuals carrying specific HLA alleles like HLA-B27 or HLA-B*51.
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Western Blot
WB result of ERAP1 Recombinant Rabbit mAb
Primary antibody: ERAP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Lane 2: unboiled mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-ERAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
