WB result of ER alpha Recombinant Rabbit mAb
Primary antibody: ER alpha Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SK-BR-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Negative control: SK-BR-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 66 kDa
ER alpha Recombinant Rabbit mAb (SDT-2950-36)
ER alpha Recombinant Rabbit mAb (SDT-2950-36)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ER alpha |
| Synonyms | Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR; NR3A1; ESR1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P03372 |
| Clone Number | SDT-2950-36 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | MCF7, T-47D |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
ER alpha (estrogen receptor alpha) is a 66-kDa ligand-activated transcription factor belonging to the nuclear receptor superfamily that, upon binding 17β-estradiol, translocates to the nucleus, homodimerizes, and recruits co-regulators to estrogen response elements (EREs) in target genes, thereby governing proliferation, differentiation, and survival in reproductive tissues, bone, cardiovascular system, and CNS, while its dysregulation drives breast and endometrial cancers, making it the principal therapeutic target of selective modulators (SERMs) and degraders (SERDs).
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 1). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer (case 2). Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ER alpha antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in T-47D cells (top panel) and negative staining in SK-BR-3 cells (below panel). Anti-ER alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
