IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin E1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Cyclin E1 Recombinant Rabbit mAb (SDT-2512-182)
Cyclin E1 Recombinant Rabbit mAb (SDT-2512-182)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cyclin E1 |
| Synonyms | G1/S-specific cyclin-E1; CCNE; CCNE1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P24864-2 |
| Clone Number | SDT-2512-182 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:100 | Ms |
Background
Cyclin E1 is a regulatory subunit of the cyclin-dependent kinase Cdk2 that drives the G1-S phase transition by phosphorylating substrates such as the retinoblastoma protein, thereby releasing E2F transcription factors and committing cells to DNA replication; its expression peaks in late G1, is tightly controlled by ubiquitin-mediated proteolysis, and is frequently amplified or overexpressed in cancers, leading to genomic instability and aggressive tumor behavior.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Cyclin E1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Cyclin E1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Cyclin E1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Cyclin E1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse testis. Anti-Cyclin E1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
