WB result of cIAP2 Recombinant Rabbit mAb
Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
cIAP2 Recombinant Rabbit mAb (S-2483-8)
cIAP2 Recombinant Rabbit mAb (S-2483-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | c-IAP2 |
| Synonyms | Baculoviral IAP repeat-containing protein 3; Apoptosis inhibitor 2 (API2); Cellular inhibitor of apoptosis 2; IAP homolog C; Inhibitor of apoptosis protein 1 (hIAP-1; hIAP1); RING finger protein 49; API2; MIHC; RNF49; BIRC3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13489 |
| Clone Number | S-2483-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Raji, Ramos, Daudi, F9, mouse spleen, mouse testis, rat testis, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
Cellular inhibitor of apoptosis protein 2 (c-IAP2, gene BIRC3) is a 618-residue member of the IAP family containing three N-terminal baculoviral IAP repeat (BIR) domains that bind caspases, a central caspase-recruitment domain (CARD) that auto-inhibits its own ubiquitin-ligase activity, and a C-terminal RING domain that endows potent E3 ubiquitin-ligase activity; through this enzymatic function c-IAP2 polyubiquitinates receptor-interacting protein 1 (RIP1) and NF-κB-inducing kinase (NIK), thereby driving canonical NF-κB prosurvival signaling while suppressing non-canonical NF-κB activation, controls TNF-α-mediated apoptosis, regulates innate immunity downstream of Toll-like and NOD-like receptors, and is frequently amplified or over-expressed in multiple cancers where its anti-apoptotic activity contributes to chemoresistance, making it a prime target for small-molecule Smac mimetics that induce its autoubiquitination and proteasomal degradation.
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Western Blot
WB result of cIAP2 Recombinant Rabbit mAb
Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
WB result of cIAP2 Recombinant Rabbit mAb
Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
WB result of cIAP2 Recombinant Rabbit mAb
Primary antibody: cIAP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-cIAP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
