WB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
CaMKII alpha Recombinant Rabbit mAb (S-2451-54)
CaMKII alpha Recombinant Rabbit mAb (S-2451-54)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CaMKII alpha |
| Synonyms | Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMKA; KIAA0968; CAMK2A |
| Immunogen | Synthetic Peptide |
| Location | Synapse |
| Accession | Q9UQM7 |
| Clone Number | S-2451-54 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, U-87 MG, mouse brain, rat brain |
| Predicted Reactivity | Or, Ck, Pg, Xe, Bv, Zf, Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
CaMKII alpha is a major isoform of Ca²⁺/calmodulin-dependent protein kinase II, predominantly expressed in the mammalian central nervous system, with high abundance in brain regions associated with learning and memory, such as the hippocampus and cerebral cortex. This protein consists of multiple catalytic domains, regulatory domains, and an oligomerization domain, typically forming 12-mer homo- or hetero-oligomeric complexes. Its core function is to decode calcium signals: when intracellular Ca²⁺ levels rise, the Ca²⁺/calmodulin complex binds to CaMKII alpha, relieving autoinhibition and activating its kinase activity. This allows it to phosphorylate various substrates, including AMPA and NMDA receptors, thereby regulating synaptic plasticity and neuronal signaling. A key characteristic is its ability to undergo "autophosphorylation" – autophosphorylation at Thr286 enables CaMKII alpha to retain partial activity even after the Ca²⁺ signal dissipates. This mechanism is considered a molecular "memory switch," crucial for long-term potentiation (LTP) and the formation of learning and memory. Furthermore, dysregulated expression or altered activity of CaMKII alpha is closely associated with various neurological disorders, such as epilepsy and Alzheimer's disease.
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Western Blot
WB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
WB result of CaMKII alpha Recombinant Rabbit mAb
Primary antibody: CaMKII alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat heart lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CaMKII alpha antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
