Flow cytometric analysis of Human CD163 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD14 antibody and either Biotin Rabbit IgG Isotype Control (Left panel) or SDT Biotin Rabbit Anti-Human CD163 Antibody (Right panel) at 5 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rabbit Anti-Human CD163 Antibody (S-222-171)
Biotin Rabbit Anti-Human CD163 Antibody (S-222-171)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD163 |
| Synonyms | Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; M130 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | Q86VB7 |
| Clone Number | S-222-171 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD163 is a type I transmembrane glycoprotein primarily expressed on the surface of monocytes and macrophages, belonging to the scavenger receptor cysteine-rich superfamily, class B. It plays a crucial role as a "clean-up" agent and immune regulator within the body. Its core function is the specific recognition and binding of the hemoglobin-haptoglobin complex, which is subsequently cleared via endocytosis by macrophages. This process protects tissues from oxidative damage caused by free hemoglobin and facilitates iron recycling. Furthermore, CD163 is recognized as a specific marker for M2 macrophages or tumor-associated macrophages. The function of CD163 is finely regulated; its membrane-bound form can be cleaved by metalloproteinases, releasing a soluble form (sCD163) into the bloodstream. Elevated levels of sCD163 serve as a biomarker for various inflammatory diseases. Recent research has also revealed that CD163 undergoes calcium concentration-dependent oligomerization via its SRCR domains, forming dimers or trimers, which is essential for its efficient ligand binding and subsequent endocytosis.
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