Flow cytometric analysis of Mouse F4/80 expression on BALB/c mouse peritoneal exudates cells. BALB/c mouse peritoneal exudates cells were stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and SDT Biotin Human Anti-Mouse F4/80 Antibody (Right panel) at 5 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Human Anti-Mouse F4/80 Antibody (S-3458)
Biotin Human Anti-Mouse F4/80 Antibody (S-3458)
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Product Details
Product Details
Product Specification
| Host | Human |
| Antigen | F4/80 |
| Synonyms | Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1 |
| Location | Cell membrane |
| Accession | Q61549 |
| Clone Number | S-3458 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse peritoneal exudates cells |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
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