Flow cytometric analysis of CD69 expression on unstimulated and stimulated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not stimulated (Left panel) or stimulated overnight (Right panel) with 3 μg/ml Concanavalin A and then stained with either PercP-Cy5.5 Armenian hamster IgG Isotype Control (Black line histogram) and SDT PercP-Cy5.5 Armenian hamster Anti-Mouse CD69 Antibody (Red line histogram) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Armenian hamster Anti-Mouse CD69 Antibody (S-R555)
Biotin Armenian hamster Anti-Mouse CD69 Antibody (S-R555)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD69 |
| Synonyms | Early activation antigen CD69; Cd69 |
| Location | Cell membrane |
| Accession | P37217 |
| Clone Number | S-R555 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | Concanavalin A stimulated Mouse splenic leukocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD69 is a type II transmembrane C-type lectin protein encoded by the CD69 gene, located on chromosome 12 in humans. It is a homodimer composed of two differentially glycosylated subunits (28–32 kDa), each containing an extracellular C-type lectin domain (CTLD), a transmembrane region, and a short cytoplasmic tail. CD69 is an early activation marker rapidly upregulated on the surface of T cells, B cells, and natural killer (NK) cells after stimulation. Its expression is regulated by transcription factors such as NF-κB, AP-1, and ERG-1. Functionally, CD69 plays a crucial role in lymphocyte proliferation, cytokine production, and retention in lymphoid tissues by interacting with molecules like S1P1 and Myl9/12. It is also implicated in the regulation of immune responses in various inflammatory and autoimmune conditions.
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