WB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDa
ATIC Rabbit Polyclonal Antibody
ATIC Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATIC |
| Synonyms | Bifunctional purine biosynthesis protein ATIC; AICAR transformylase/inosine monophosphate cyclohydrolase (ATIC); PURH |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P31939 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, HT-29, NIH/3T3, C6 |
| Predicted Reactivity | Or, Bv |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
Background
ATIC protein, also known as 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase, is a bifunctional enzyme that catalyzes the last two steps in the de novo purine biosynthetic pathway. The N-terminal domain of ATIC possesses phosphoribosylaminoimidazolecarboxamide formyltransferase activity, while the C-terminal domain has IMP cyclohydrolase activity . Mutations in the ATIC gene can lead to AICA-ribosiduria, a condition characterized by the accumulation of AICA riboside and its derivatives in the body.
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Western Blot
WB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDa
WB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
