Flow cytometric analysis of Mouse Ter-119 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with PE-Cy7 Rat Anti-Mouse CD45 Antibody and either Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (Left panel) or Alexa Fluor® 488 Rat Anti-Mouse TER-119/Erythroid Cells Antibody (Right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse TER-119/Erythroid Cells Antibody (S-R517)
Alexa Fluor® 488 Rat Anti-Mouse TER-119/Erythroid Cells Antibody (S-R517)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse Ter-119/Erythroid Cells |
| Synonyms | Lymphocyte antigen 76; Ly76; Ly-76; Ter119 |
| Location | Cell membrane |
| Clone Number | S-R517 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
The TER-119 protein is one of the most specific and important cell surface markers for the murine erythroid lineage. It specifically recognizes an antigen associated with glycophorin A on the red blood cell membrane. Throughout erythroid development, from early basophilic erythroblasts to late-stage reticulocytes, it is consistently highly expressed, making it a critical molecular marker for identifying, sorting, and studying murine erythroid cells (such as erythrocytes, progenitor cells, and precursors). This protein belongs to the Ly-6 antigen superfamily, and its antibody (e.g., the TER-119 monoclonal antibody) is an indispensable tool in experimental hematology for the isolation and identification of red blood cells via flow cytometry or immunomagnetic beads, as well as for research into blood diseases like anemia and leukemia.
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