Recombinant Cytokines

Recombinant cytokines are engineered soluble signaling proteins that mediate intercellular immune communication, serving as irreplaceable foundational tools across in vitro cell culture, immunological functional assays, and preclinical in vivo animal studies. Reliable research-grade cytokine products must satisfy four core quality criteria:

• High Bioactivity: Matched to native endogenous cytokine potency, validated via cell proliferation or reporter functional bioassays
• High Purity: Over 95% purity verified by SDS-PAGE and HPLC, free of contaminating host cell proteins
• Low Endotoxin: Endotoxin levels below 0.1 EU/μg to eliminate adverse effects on sensitive primary and immune cells
• Lot-to-Lot Consistency: Uniform activity and purity across production batches to guarantee long-term experimental repeatability

Core Research Applications

1. Immune Cell Culture, Differentiation & Expansion

1. Use GM-CSF and M-CSF to induce monocyte differentiation into dendritic cells (DCs) and macrophages for antigen presentation research
2. Apply IL-2, IL-4 and IL-6 to regulate T helper cell polarization and support T/B lymphocyte survival for CAR-T and hybridoma culture
3. Utilize TGF-β1 to drive Treg cell differentiation for immune tolerance and tumor microenvironment modeling

2. Immune Activation & Functional Phenotyping Assays

1. Stimulate immune cells with IFN-γ, TNF-α and IL-1β to construct inflammation, autoimmunity and host-pathogen interaction models
2. Conduct quantitative effector function detection via ELISA, flow cytometry and transcriptomic profiling after cytokine stimulation
3. Co-culture EGF with immune cytokines to investigate crosstalk between immune cells and epithelial/tumor cells in tumor immunology

3. Preclinical In Vivo Animal Administration

1. Inject low-endotoxin cytokines into mouse/rat models to recapitulate physiological or pathological immune states
2. Establish disease models including cytokine storm, inflammatory disorders and tumor immune suppression
3. Adopt consistent batch proteins to eliminate variability between animal experimental cohorts

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