WB result of S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb
Primary antibody: S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-239 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb
S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Sulfo-Serine/Threonine |
| Immunogen | Synthetic Peptide |
| Antibody Type | Recombinant mAb Mix |
| Isotype | IgG |
| Application | ELISA, WB |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Species independent |
Background
Sulfo-Serine/Threonine refers to the process of sulfation modification occurring on serine (Ser) or threonine (Thr) residues of proteins, which is a post-translational modification that adds a sulfo group (-SO₃H) to the hydroxyl group of the amino acid residue.
Picture
Picture
Western Blot
WB result of S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb
Primary antibody: S-RMabMix™ Sulfo-Serine/Threonine Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Lane 4: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
This blot was developed with high sensitivity substrate
ELISA
ELISA binding assay shows Sulfo Serine/Threonine Rabbit monoclonal antibody specifically binds to sulfo serine and threonine modified peptide.
