Product Introduction: Multiplex Fluorescence IHC Staining Kits for Tumor Microenvironment Research

1. Background Introduction

Immunohistochemical staining is a common technique for studying tissue morphology and in situ protein expression. With the rise of immunotherapy, an increasing number of markers in the tumor microenvironment have been found to be closely related to disease treatment and progression. Simultaneous staining and labeling of these markers, as well as research on their colocalization, expression levels, and distance relationships, have become universal needs in tumor immunology research.

The multiplex staining kits from the Absin product line of ANT BIO PTE. LTD. optimize the combination of antibodies and fluorescent dyes using Tyramide Signal Amplification (TSA) technology, developing a series of fluorescent immunohistochemical kits that can simultaneously label and detect multiple biomarkers.

2. Product Overview

The tumor microenvironment consists of tumor cells, cancer-associated fibroblasts, immune cells, secretory products of the above cells (such as cytokines and chemokines), and non-cellular components in the extracellular matrix. The Tumor Microenvironment Series Staining Kits can label keratin (pan-CK), T cells, myeloid-derived immune cells, and immune checkpoint proteins. Each kit allows the selection of any 3 markers from the above.

3. Application

It is mainly used for immunofluorescent staining of Tumor-Infiltrating Lymphocytes (TILs) in the tumor microenvironment research series.

4. Technical Principle

Tyramide Signal Amplification (TSA) technology uses HRP-labeled secondary antibodies. HRP catalyzes the fluorescent substrate added to the system to generate activated fluorescent substrates. The activated substrates can covalently bind to tyrosine on the antigen, enabling stable covalent binding of fluorescein on the sample. Subsequently, heat-induced epitope retrieval is used to wash away non-covalently bound antibodies, followed by incubation with the next primary antibody in the second round and replacement with another fluorescent substrate. This cycle is repeated to achieve multiplex labeling.

5. Experimental Flow Chart

Sample Preparation → Incubation with Primary Antibody 1 → TSA Reaction (Dye 1) → Antibody Elution → Incubation with Primary Antibody 2 → TSA Reaction (Dye 2) → Antibody Elution → Incubation with Primary Antibody 3 → TSA Reaction (Dye 3) → Antibody Elution → DAPI Staining → Imaging

6. Product Advantages

•         High Scientific Research Value: Available in both human and mouse series, facilitating parallel experiments on mouse tumor models and human samples, and supporting translational medicine research.

•         High Compatibility: The selected fluorescent dye labels are suitable for all fluorescence microscopes, confocal microscopes, and fluorescence section scanning equipment equipped with ultraviolet, FITC, CY3, CY5, and other wavelength bands.

•         Stability: No light protection is required during the sample addition process. After staining, the samples can be stored at 4°C for half a year and at -20°C for one year, and the fluorescent signals still meet the photography requirements.

•         Convenience: All antibodies have been screened for good specificity, and the staining concentrations have been optimized, reducing the process of researchers optimizing conditions by themselves.

7. Product Recommendation

8. Brand Mission

ANT BIO PTE. LTD. is dedicated to empowering life science research through the provision of high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) are strategically positioned to cover a full spectrum of research needs: Absin focuses on general reagents and kits, Starter specializes in antibodies, and UA is dedicated to recombinant proteins. We strive to support researchers in unlocking scientific mysteries, advancing medical progress, and addressing unmet clinical needs through continuous innovation and customer-centric services.

9. Disclaimer

This article is AI-compiled and interpreted based on the original work in document 1226.docx (Multiplex Fluorescence IHC Staining Kits). All product-related data and technical information are for research purposes only and are not intended for clinical diagnosis or treatment. For any product quality issues, please contact us promptly and we will handle them actively.

10. Brand Promotion Copy

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.