Comprehensive Protein Purification Solutions by ANT BIO PTE. LTD.: High Capacity, Resolution & Recovery
1. Overview of Protein Purification Solutions
Protein purification is a fundamental and critical step in life science research, biopharmaceutical development, and industrial biotechnology. ANT BIO PTE. LTD. is dedicated to providing comprehensive protein purification solutions characterized by high binding capacity, high resolution, and high recovery rate. Our solutions cover the entire purification workflow from crude purification to fine purification, offering tailored resins and supporting tools for each stage. With excellent compatibility and professional technical support, we effectively assist researchers and industry professionals in optimizing purification processes and improving research and production efficiency.
1.1 Core Advantages of Our Solutions
• Full Workflow Coverage: From crude purification to fine purification, we provide suitable resins and supporting tools for each stage, enabling seamless connection of the entire purification process.
• High Compatibility: Compatible with prokaryotic/eukaryotic expression systems, various tag types, and complex samples, meeting diverse purification needs in different research and production scenarios.
• Professional Technical Support: Our professional technical team provides customized solutions and one-on-one technical guidance to help users optimize purification processes and solve practical problems encountered in experiments.
2. Detailed Product Portfolio of Protein Purification Solutions
ANT BIO PTE. LTD.'s protein purification solutions cover a complete range of products, including antibody affinity purification resins, tagged protein purification resins, ion exchange purification resins, hydrophobic & multimodal resins, and specific affinity purification resins. Each product is meticulously developed and optimized to ensure outstanding performance in specific purification scenarios.
2.1 Antibody Affinity Purification Resins
Our antibody affinity purification resins enable the acquisition of high-purity antibodies through a single purification step, suitable for the efficient purification of various samples such as ascites, serum, and cell culture supernatants. The purification workflow of antibody affinity resins typically includes equilibration, sample loading, washing, elution, and regeneration (CIP). For detailed product solutions, please refer to: "Efficient Antibody Purification: In-depth Analysis of Recombinant Protein A, G, A/G, L Resin Technology".
Recommended products for antibody affinity purification are shown in the following table:
|
Purification Method |
Reagent Type |
Product Type |
Recommended Products (Cat. No.) |
|
Antibody Affinity Purification |
Recombinant Protein A Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Recombinant Protein A Sepharose Gel FF (abs91001) |
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Protein A Sepharose Magnetic Beads (abs9904) |
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Recombinant Protein G Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Recombinant Protein G Sepharose Gel FF (abs91002) |
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Alkali-Resistant Protein A Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Alkali-Resistant Protein A Sepharose Gel FF (abs91003) |
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Protein A/G Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Protein A/G Sepharose Gel FF (abs91004) |
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Protein A/G Sepharose Magnetic Beads (abs90323) |
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Protein A/G Magnetic Beads (abs90146) |
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Easy-Elution Protein G Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Easy-Elution Protein G Sepharose Gel FF (abs91005) |
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Recombinant Protein L Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Recombinant Protein L Sepharose Gel FF (abs91006) |
2.2 Tagged Protein Purification Resins
Our tagged protein purification resins support multiple tags including His, GST, MBP, and Strep II, meeting diverse purification needs of different tagged proteins. Taking the Strep II Sepharose Gel FF for purifying Strep II-tagged proteins as an example, its purification workflow includes: 1) Equilibration; 2) Sample loading and binding; 3) Elution with D-biotin; 4) Regeneration (using 10~50 mM NaOH). For detailed product solutions, please refer to: "Comprehensive Analysis of Tagged Protein Purification Resins: In-depth Guide from Principle to Application".
Recommended products for tagged protein purification are shown in the following table:
|
Purification Method |
Reagent Type |
Product Type |
Recommended Products (Cat. No.) |
|
Tagged Protein Purification |
His-Tagged Protein Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Ni-IDA Sepharose Gel FF (abs91007) |
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Ni-NTA Sepharose Gel FF (abs91008) |
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Ni-TED Sepharose Gel FF (abs91009) |
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His-Tag Protein Purification Magnetic Beads (IDA-Ni) (abs90154) |
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His-Tag Protein Purification Magnetic Beads (NTA-Ni) (abs90156) |
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His-Tag Protein Purification Magnetic Beads (TED-Ni) (abs90326) |
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GST-Tagged Protein Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
GST Sepharose Gel FF (abs91010) |
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GST Fusion Protein Purification Magnetic Beads (abs90159) |
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MBP-Tagged Protein Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
MBP Sepharose Gel FF (abs91011) |
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Biotin-Tagged Protein Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Biotin Sepharose Gel FF (abs91012) |
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Biotin-Tagged Protein Purification Magnetic Beads (abs90328) |
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Strep II-Tagged Protein Purification |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Strep II Sepharose Gel FF (abs91013) |
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Strep-Tag II Protein Purification Magnetic Beads (abs90162) |
2.3 Ion Exchange Purification Resins
Our ion exchange purification resins are characterized by high dynamic binding capacity and excellent selectivity, ensuring efficient capture and fine purification of target molecules with broad applicability. The purification principle is based on the difference in surface charge of proteins: when the pH of the solution is lower than the isoelectric point (pI) of the protein, the protein carries a positive charge and binds to the anion exchange resin; when the pH is higher than the pI, the protein carries a negative charge and binds to the cation exchange resin. By adjusting the pH and ionic strength of the elution buffer, different proteins can be eluted sequentially to achieve effective separation and purification.
Recommended products for ion exchange purification are shown in the following table:
|
Purification Method |
Reagent Type |
Product Type |
Recommended Products (Cat. No.) |
|
Ion Exchange Purification |
Anion Exchange Chromatography |
Resin, Medium Pressure Column, Gravity Column |
Q Anion Exchange Sepharose Gel FF (abs91014) |
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Q Anion Exchange High-Resolution Sepharose Gel HP (abs91015) |
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DEAE Anion Exchange Sepharose Gel FF (abs91016) |
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Cation Exchange Chromatography |
Resin, Medium Pressure Column, Gravity Column |
SP Cation Exchange Sepharose Gel FF (abs91017) |
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CM Cation Exchange Sepharose Gel (abs91018) |
2.4 Hydrophobic & Multimodal Resins
We offer hydrophobic and multimodal resins with a variety of ligand types and hydrophobic strengths, providing different selectivities for protein purification. The clever combination of multiple interaction modes (such as hydrophobic interaction, ion exchange, and hydrogen bonding) in multimodal resins brings novel selectivity and a wider range of operating conditions, making them suitable for the purification of complex samples and difficult-to-separate proteins. Typical multimodal resins include Anion Exchange Multimodal Sepharose Gel Adhere (integrating anion exchange, hydrophobic interaction, and hydrogen bonding), Cation Exchange Multimodal Sepharose Gel MMC (integrating cation exchange and hydrophobic interaction), and Octylamine Core-Shell Sepharose Gel Core (with a ~5μm inert shell for gel filtration and octylamine core for hydrophobic interaction).
Recommended products for hydrophobic & multimodal purification are shown in the following table:
|
Purification Method |
Reagent Type |
Product Type |
Recommended Products (Cat. No.) |
|
Hydrophobic & Multimodal Purification |
Hydrophobic Interaction Chromatography |
Resin, Medium Pressure Column, Gravity Column |
Butyl Sepharose Gel FF (abs91019) |
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Phenyl High-Substitution Sepharose Gel FF (abs91020) |
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Phenyl Low-Substitution Sepharose Gel FF (abs91021) |
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Octyl Hydrophobic Sepharose Gel FF (abs91022) |
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Cation/Anion Multimodal |
Resin, Medium Pressure Column, Gravity Column |
Anion Exchange Multimodal Sepharose Gel Adhere (abs91023) |
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Cation Exchange Multimodal Sepharose Gel MMC (abs91024) |
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2-Mercaptopyridine High-Resolution Sepharose Gel HP (abs91025) |
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Octylamine Core-Shell Sepharose Gel Core (abs91026) |
2.5 Specific Affinity Purification Resins
Our specific affinity purification resins are equipped with specific group ligands, enabling targeted purification of target proteins. Recommended products for specific affinity purification are shown in the following table:
|
Purification Method |
Reagent Type |
Product Type |
Recommended Products (Cat. No.) |
|
Specific Affinity Purification |
Specific Group Ligand |
Resin, Medium Pressure Column, Gravity Column, Magnetic Beads |
Heparin Sepharose Gel FF (abs91035) |
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Heparin Magnetic Beads (abs90164) |
ANT BIO PTE. LTD.'s protein purification solutions are widely applicable to various fields, including:
• Basic Life Science Research: Purification of recombinant proteins, antibodies, enzymes, and other biomolecules for structural and functional studies.
• Biopharmaceutical Development: Purification of therapeutic proteins, monoclonal antibodies, and vaccine antigens, meeting the strict quality requirements of biopharmaceutical production.
• Clinical Diagnosis: Purification of diagnostic reagents and biomarkers, ensuring the accuracy and reliability of diagnostic results.
• Industrial Biotechnology: Purification of industrial enzymes and other biocatalysts, improving the efficiency and quality of industrial production.
ANT BIO PTE. LTD. is committed to providing not only high-quality protein purification products but also comprehensive technical support and services. Our professional technical team has rich experience in protein purification and can provide customized solutions according to the specific needs of users, including sample analysis, resin selection, process optimization, and troubleshooting. In addition, we provide detailed product manuals and experimental protocols to help users quickly get started with the products and achieve ideal purification results.
ANT BIO PTE. LTD. is dedicated to advancing life science research and biomanufacturing by providing high-quality, reliable reagents and comprehensive solutions. Our protein purification solutions are an important part of our product portfolio, designed to solve the pain points and challenges in protein purification processes for users. Guided by the principles of innovation, quality, and customer-centricity, our three specialized sub-brands (Absin, Starter, and UA) cover a full spectrum of research and production needs, from general reagents and kits to purification resins and equipment. We strive to establish long-term and trusted partnerships with users worldwide, supporting them in achieving breakthroughs in research and production and contributing to the development of the biotechnology industry.
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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs & Biomanufacturing Excellence
At ANTBIO, we are committed to advancing life science research and biomanufacturing through high-quality, reliable reagents and comprehensive solutions. Our comprehensive protein purification solutions, with high capacity, resolution, and recovery rate, cover the entire purification workflow and meet diverse application needs. Backed by professional technical support and customized services, we strive to be your trusted partner in unlocking scientific mysteries and driving biomanufacturing progress. Explore our product portfolio today and elevate your protein purification processes to new heights.
