Protein Purification Magnetic Beads/Kits by ANT BIO PTE. LTD.: Efficient Capture of Target Molecules

1. Experimental Principle

Protein purification magnetic beads/kits achieve precise capture and purification of target molecules based on specific biomolecular interactions. The surface of the magnetic beads is modified with high-affinity ligands (such as antibodies, streptavidin, His-tag binding groups, etc.), which can specifically bind to target proteins. For example, antibody-conjugated magnetic beads selectively adsorb target proteins through the antigen-antibody binding principle, while streptavidin magnetic beads capture biotinylated molecules using the high affinity between biotin and avidin.

After binding, the magnetic bead-target molecule complex is quickly separated from impurities through magnetic separation technology. Non-specific adsorption is then removed by washing, and finally, high-purity target products are released by changing buffer conditions (such as pH adjustment, competitive elution). This technology combines the advantages of high efficiency, mildness, and automation, and is suitable for the purification of trace targets in complex samples (such as serum, cell lysates).

2. Experimental Protocol (Taking Antibody-Conjugated Magnetic Beads as an Example)

2.1 Sample Pretreatment

•         Centrifugation for Impurity Removal: Centrifuge the sample (e.g., serum) at 3000g for 10 minutes to remove cell debris or particles.

•         Lysis (Optional): If the target is an intracellular component, treat the sample with lysis buffer (containing protease inhibitors), perform ultrasonic disruption, and centrifuge to collect the supernatant.

2.2 Magnetic Bead Incubation

•         Magnetic Bead Activation: Vortex to mix the magnetic bead suspension thoroughly, take an appropriate amount of magnetic beads into a centrifuge tube, and wash twice with pre-cooled PBS to remove the storage solution.

•         Binding of Target Molecules: Mix the pretreated sample with magnetic beads, and incubate with rotation at room temperature for 30 minutes to ensure sufficient contact.

2.3 Magnetic Separation and Washing

•         Magnetic Attraction to Remove Supernatant: Place the centrifuge tube on a magnetic stand and let it stand for 2-3 minutes. After the solution becomes clear, aspirate and discard the supernatant.

•         Washing Impurities: Add washing buffer (e.g., PBS containing 0.1% Tween-20), gently resuspend the magnetic beads, and repeat the magnetic attraction step 2-3 times to remove non-specific binding.

2.4 Elution of Target Molecules

Competitive or Conditional Elution:

•         Acidic Elution: Add low-pH buffer (e.g., pH 2.8 glycine-HCl), incubate for 5 minutes, neutralize immediately, and transfer the supernatant to protect the target activity.

•         Biotin Competition: For streptavidin magnetic beads, add excess free biotin to competitively dissociate target molecules.

Magnetic Bead Regeneration (Optional): Some kits support magnetic bead regeneration, which can be reused after treatment with deionized water and regeneration buffer.

Total Time Consumption: Approximately 60 minutes, inter-batch variation < 5%, and recovery rate up to 90% or more.

3. Product Advantages

•         Release microspheres, breaking free from the limitations of sample volume, reagent flow rate, chromatography column pressure, and elution volume.

•         Simultaneously achieve solid-liquid and liquid-liquid separation, simplifying steps, reducing losses, and saving time.

•         Break through the technical bottlenecks in the purification of secreted proteins and inclusion body proteins.

•         Reduce reliance on instrumentation and equipment, lowering maintenance and upkeep costs.

•         Stable operation, facilitating high-throughput and large-scale protein purification.

•         Stable product performance with small batch-to-batch variation.

•         Achieve high yield and high purity of target proteins.

4. Related Products

ANT BIO PTE. LTD. provides a complete series of protein purification magnetic beads and kits to meet diverse experimental needs. The detailed product information is shown in the following table:

5. Brand Mission

ANT BIO PTE. LTD. is dedicated to advancing life science research by providing high-quality, reliable protein purification reagents and comprehensive solutions. Our protein purification magnetic beads and kits are carefully developed and optimized to solve the pain points of complex sample purification and low recovery rate for researchers. Guided by the principles of innovation, quality, and customer-centricity, our three specialized sub-brands (Absin, Starter, and UA) cover a full spectrum of research needs, from general reagents to specialized purification kits.

We strive to establish long-term and trusted partnerships with researchers worldwide, supporting them in achieving breakthroughs in protein research and contributing to the development of life science fields such as immunology, oncology, and neuroscience.

6. Disclaimer

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7. Brand Promotion Copy

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our protein purification magnetic beads and kits, with efficient capture capacity and stable performance, simplify purification processes and ensure high yield and purity of target proteins. Backed by professional technical support, we strive to be your trusted partner in unlocking scientific mysteries. Explore our product portfolio today and elevate your protein purification experiments to new heights.