One-Stop Protein Purification Solutions by ANT BIO PTE. LTD.: High Capacity, Resolution & Recovery
1. Overview of One-Stop Protein Purification Solutions
Protein purification is a critical step in life science research, drug development, and biomanufacturing. To meet the diverse needs of researchers and industry professionals in protein purification processes, ANT BIO PTE. LTD. launches a comprehensive one-stop protein purification solution. This solution features high binding capacity, high resolution, and high recovery rate, covering the entire purification workflow from crude purification to fine purification, and provides tailored fillers and supporting tools for each stage. With strong compatibility and professional technical support, it effectively helps optimize purification processes and improve research and production efficiency.
• Full Process Coverage: From crude purification to fine purification, the solution provides suitable fillers and supporting tools for each stage, realizing a seamless connection of the entire purification process.
• High Compatibility: Compatible with prokaryotic/eukaryotic expression systems, various tag types, and complex samples, meeting the diverse purification needs of different research and production scenarios.
• Professional Support: The professional technical team of ANT BIO PTE. LTD. provides customized solutions, offering one-on-one technical guidance to help users optimize purification processes and solve practical problems encountered in experiments.
2. Product Portfolio of One-Stop Protein Purification Solutions
ANT BIO PTE. LTD.'s one-stop protein purification solution covers a complete range of purification fillers, including antibody affinity purification fillers, tagged protein purification fillers, ion exchange purification fillers, hydrophobic & multimodal fillers, and gel filtration fillers. Each type of filler is carefully developed and optimized to ensure excellent performance in specific purification scenarios.
2.1 Antibody Affinity Purification Resins
The antibody affinity purification resins of ANT BIO PTE. LTD. enable high-purity antibody acquisition through a single purification step. They are suitable for high-efficiency purification of various samples such as ascites, serum, and cell culture supernatants. The resins feature high specificity and strong binding capacity, which can specifically capture target antibodies while minimizing non-specific adsorption, ensuring high recovery rate and purity of the purified antibodies. This product greatly simplifies the antibody purification process, saving time and labor costs for researchers.
2.2 Tagged Protein Purification Resins
To meet the purification needs of different tagged proteins, ANT BIO PTE. LTD. provides tagged protein purification resins that support multiple tags including His, GST, MBP, and Strep II. These resins are designed for specific tag-protein interactions, ensuring high selectivity and binding efficiency. Taking the Strep II Agarose Gel FF for Strep II-tagged protein purification as an example, the purification process follows a standardized workflow: 1) Equilibration; 2) Sample loading and binding; 3) Elution with D-biotin; 4) Regeneration (using 10~50 mM NaOH). The simple and efficient operation ensures the rapid purification of target proteins with high purity.
2.3 Ion Exchange Purification Resins
The ion exchange purification resins of ANT BIO PTE. LTD. are characterized by high dynamic binding capacity and excellent selectivity, enabling efficient capture and fine purification of target molecules with broad applicability. The purification principle is based on the difference in surface charge of proteins. When the pH of the solution is less than the isoelectric point (pI) of the protein, the protein carries a positive charge and binds to the anion exchange resin; when the pH is greater than the pI, the protein carries a negative charge and binds to the cation exchange resin. By adjusting the pH and ionic strength of the elution buffer, different proteins can be sequentially eluted, achieving effective separation and purification.
2.4 Hydrophobic & Multimodal Resins
ANT BIO PTE. LTD. offers hydrophobic and multimodal resins with a variety of ligand types and hydrophobic strengths, providing different selectivities for protein purification. The clever combination of multiple interaction modes (such as hydrophobic interaction, ion exchange, and hydrogen bonding) in multimodal resins brings novel selectivity and a wider range of operating conditions, making them suitable for the purification of complex samples and difficult-to-separate proteins.
Key product types include: Anion Exchange Multimodal Agarose Gel Adhere (integrating anion exchange, hydrophobic interaction, and hydrogen bonding), Cation Exchange Multimodal Agarose Gel MMC (integrating cation exchange and hydrophobic interaction), and Octylamine Core-Shell Agarose Gel Core (with a 5μm inert shell for gel filtration and octylamine core for hydrophobic interaction).
The gel filtration resins of ANT BIO PTE. LTD. have precise exclusion limits and low non-specific adsorption, ensuring high recovery rates in desalting and buffer exchange operations. The purification principle is based on the molecular sieve effect: when the sample passes through the gel column, molecules with different sizes are separated according to their ability to enter the gel pores. Small molecules can enter the pores and have a longer retention time, while large molecules cannot enter the pores and elute first. This product is widely used in the final polishing step of protein purification, as well as desalting and buffer exchange of samples.
ANT BIO PTE. LTD.'s one-stop protein purification solutions are widely applicable to various fields, including:
• Basic Life Science Research: Purification of recombinant proteins, antibodies, enzymes, and other biomolecules for structural and functional studies.
• Biopharmaceutical Development: Purification of therapeutic proteins, monoclonal antibodies, and vaccine antigens, meeting the strict quality requirements of biopharmaceutical production.
• Clinical Diagnosis: Purification of diagnostic reagents and biomarkers, ensuring the accuracy and reliability of diagnostic results.
• Industrial Biotechnology: Purification of industrial enzymes and other biocatalysts, improving the efficiency and quality of industrial production.
ANT BIO PTE. LTD. is committed to providing not only high-quality protein purification products but also comprehensive technical support and services. Our professional technical team has rich experience in protein purification and can provide customized solutions according to the specific needs of users, including sample analysis, filler selection, process optimization, and troubleshooting.
In addition, we provide detailed product manuals and experimental protocols to help users quickly get started with the products and achieve ideal purification results.
ANT BIO PTE. LTD. is dedicated to advancing life science research and biomanufacturing by providing high-quality, reliable reagents and comprehensive solutions. The one-stop protein purification solution is an important part of our product portfolio, designed to solve the pain points and challenges in protein purification processes for users. Guided by the principles of innovation, quality, and customer-centricity, our three specialized sub-brands (Absin, Starter, and UA) cover a full spectrum of research and production needs, from general reagents and kits to purification fillers and equipment. We strive to establish long-term and trusted partnerships with users worldwide, supporting them in achieving breakthroughs in research and production and contributing to the development of the biotechnology industry.
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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs & Biomanufacturing Excellence
At ANTBIO, we are committed to advancing life science research and biomanufacturing through high-quality, reliable reagents and comprehensive solutions. Our one-stop protein purification solutions, with high capacity, resolution, and recovery rate, cover the entire purification workflow and meet diverse application needs. Backed by professional technical support and customized services, we strive to be your trusted partner in unlocking scientific mysteries and driving biomanufacturing progress. Explore our product portfolio today and elevate your protein purification processes to new heights.
