Ready-to-Use Multicolor Flow Cytometry Mini Panels to Simplify Immune Subset Analysis
1. Overview
AntBio MultiColor Flow Cytometry Mini Panel Kits are pre-optimized, ready-to-use reagents for multicolor flow immunophenotyping. Validated for human and mouse samples, they include 10+ classic immune panels for comprehensive immune cell identification, polarization profiling and functional subset quantification. All fluorophore combinations, antibody cocktails and staining protocols are fully verified. Streamlined, highly reproducible assays are achievable with peripheral blood, spleen, bone marrow and various tissue samples, suited for basic immunology and preclinical pharmacodynamic studies.
Panel List
Table 1: Product Catalog & Application Scenario List
| Cat. No. | Product Name | Application Scenario |
|---|---|---|
| S0N0001 | Mouse MacroPhage Panel Kit | Mouse Macrophage Phenotyping |
| S0N0002 | Mouse NK Cell Panel Kit (CD45/CD3/CD49b) | Mouse NK Cell Assay |
| S0N0003 | Mouse T Cell Panel kit | Mouse T Cell Immunophenotyping |
| S0N0004 | Human B Cell Panel Kit | Human B Cell Subset Analysis |
| S0N0005 | Mouse B Cell Panel Kit | Human B Cell Subset Analysis |
| S0N0006 | Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD56/CD14) | Human Immune Subset Profiling |
| S0N0007 | Human T + B + NK + Monocyte Panel Kit (CD3/CD4/CD8/CD19/CD16/CD14) | Human Immune Subset Profiling |
| S0N0008 | Mouse DC Panel Kit | Mouse Dendritic Cells Assay |
| S0N0009 | Human Tissue-Resident T Cells Kit | Human Tissue-Resident T Cells Analysis |
| S0N0010 | Human MLR Panel Kit | Mixed Lymphocyte Reaction Monitoring |
| S0N0011 | Human DC Panel Kit | Human Dendritic Cells Phenotyping |
| S0N0012 | Mouse TE/TN/TCM/TEM Panel Kit | Mouse T Cell Differentiation Profiling |
| S0N0013 | Mouse Myeloid Panel Kit | Mouse Myeloid Subset Analysis |
| S0N0014 | Mouse NK Cell Panel Kit (CD45/CD3/NK1.1) | Mouse NK Cell Assay |
2. Immunophenotyping Panels
2.1 Macrophage Panel
Macrophages are tissue-resident leukocytes differentiated from circulating monocytes, which originate from hematopoietic progenitor cells in the bone marrow. As professional phagocytes, both monocytes and macrophages participate in innate immunity (non-specific defense) and cell-mediated adaptive immunity (specific defense) in vertebrates. Existing as either sessile tissue-resident cells or migratory circulating cells, they execute phagocytosis to eliminate apoptotic cells, cellular debris and invading pathogens. Additionally, they activate lymphocytes and other immune cells to accelerate host immune responses against pathogens.
Note: This protocol outlines a universal detection strategy for total macrophages. Supplementary phenotypic markers are required to distinguish monocyte-derived infiltrating macrophages from tissue-resident macrophage subsets.
Figure 2. Macrophage marker profile: F4/80*,CD11b+, CD86+, CD206, CD80+, CD163, CD11C+, INOS
Table 2: S0N0001 Mouse Macrophage Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0001 | Mouse Macrophage Panel Kit |
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) |
| S0B5160-P | PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70) |
| S0B5458-P | PE Rat Anti-Mouse F4/80 Antibody (S-R537) |
| S0B1673-P | Alexa Fluor® 488 Rat Anti-Mouse CD86/B7-2 Antibody (GL-1) |
| S0B5331-P | Alexa Fluor® 647 Rat Anti-mouse CD206 (MMR) Antibody (S-R498) |
| S0B1700-P | Alexa Fluor® 700 Rat Anti-Mouse Ly6G Antibody (1A8) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 545 (Cat. S0B88802) to perform live/dead discrimination.
Figure 3. S0N0001 Mouse Macrophage Panel Kit
multi-sample validation gating plots
2.2 Natural Killer (NK) Cell Panel
NK cells are a major subset of innate lymphoid cells (ILCs) distinguished from adaptive T and B lymphocytes by lacking somatically rearranged antigen receptors encoded by recombination-activation genes. These large granular lymphocytes eliminate infected and tumor cells without pre-sensitization through coordinating activating and inhibitory receptors. Activated NK cells release perforin, granzymes and IFN-γ to kill target cells and modulate immune responses. Originating from bone marrow stem cells, they possess tissue-specific subsets and have promising applications in cancer immunotherapy.
Figure 4. NK cell gating logic schematic (CD45+ lymphoid subset classification)
Table 3: S0N0002 Mouse NK Cell Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0002 | Mouse NK Cell Panel Kit |
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) |
| S0B1783-P | FITC Armenian hamster Anti-Mouse CD3 Antibody (S-R491) |
| S0B5751-P | APC Rat Anti-Mouse CD49b Antibody (DX5) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 452 (S0B88801) to perform live/dead discrimination.
Figure 5. S0N0002 Mouse NK Cell Panel Kit
multi-sample validation gating plots
2.3 T Cell Panel
T cells are thymus-matured adaptive immune lymphocytes with TCRs for MHC-restricted antigen recognition. CD4⁺ helper T cells regulate immune responses, while CD8⁺cytotoxic T cells kill abnormal cells. Memory T cells provide lasting protective immunity, and T cell-based therapies are widely studied for cancer treatment.
Figure 6. T cell subset classification schematic (CD3+ T cell subgroups)
Table 4: S0N0003 Mouse T Cell Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0003 | Mouse T Cell Panel Kit |
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) |
| S0B1783-P | FITC Armenian hamster Anti-Mouse CD3 Antibody (S-R491) |
| S0B5133-P | PerCP-Cy5.5 Rat Anti-Mouse CD4 Antibody (GK1.5) |
| S0B5086-P | APC Rat Anti-Mouse CD8α Antibody (53-6.7) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 452 (S0B88801) to perform live/dead discrimination.
Figure 7. S0N0003 Mouse T Cell Panel Kit
multi-sample validation gating plots
2.4 B Cell Panel
B cells are key adaptive immune cells that mature in bone marrow. They have unique rearranged B cell receptors (BCRs) to bind foreign antigens. When stimulated by antigens, B cells multiply and turn into plasma cells that make antibodies for humoral immunity; some become long-lived memory B cells for fast secondary protection. Aside from making antibodies, B cells present antigens to activate T cells and release cytokines to regulate immune reactions. Dysfunctional B cells cause autoimmune diseases, so they are important targets for immune disease therapies.
Figure 8. B cell developmental trajectory schematic
Table 5: S0N0004 Human B Cell Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0004 | Human B Cell Panel Kit |
| S0B1527-P | FITC Mouse Anti-Human CD19 Antibody (S-1039-46) |
| S0B5440-P | Pacific Blue Mouse Anti-Human CD20 Antibody (2H7) |
| S0B5646-P | PE Mouse Anti-Human CD138 Antibody (MI15) |
| S0B5334-P | Alexa Fluor® 647 Mouse Anti-Human CD24 Antibody (ML5) |
| S0B5734-P | PE-Cy7 Mouse Anti-Human CD38 Antibody (S-R507-1) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 777 (S0B88805) to perform live/dead discrimination.
Figure 9. S0N0004 Human B Cell Panel Kit
validation gating plots
Table 6: S0N0005 Mouse B Cell Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0005 | Mouse B Cell Panel Kit |
| S0B5627-P | Pacific Blue Rat Anti-Mouse CD45R/B220 Antibody (RA3-6B2) |
| S0B5060-P | APC-Cy7 Rat Anti-Mouse CD19 Antibody (1D3) |
| S0B1511-P | Alexa Fluor® 488 Mouse Anti-Mouse CD20 Antibody (18B12) |
| S0B5132-P | PE-Cy7 Rat Anti-Mouse CD38 Antibody (90) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 583 (S0B88803) to perform live/dead discrimination and 0.5 μg APC Rat Anti-Mouse CD45 Antibody (30-F11) (S0B1676) for leukocyte discrimination.
Figure 10. S0N0005 Mouse B Cell Panel Kit
multi-sample validation gating plots
2.5 T+ B+ NK+ Monocyte Panel
This six-color panel is designed for detecting T cells, B cells, NK cells, and monocytes in human peripheral blood or other biological samples. The panel is compatible with most three-laser flow cytometers. It acts as a core backbone panel — users may add fluorophore-conjugated antibodies to detect any additional markers of interest as needed.
Table 7: S0N0006 Human T+B+NK+Monocyte Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0006 | Human T + B + NK + Monocyte Panel Kit |
| S0B5572-P | APC-Cy7 Mouse Anti-Human CD3 Antibody (HIT3a) |
| S0B1606-P | PerCP-Cy5.5 Mouse Anti-Human CD4 Antibody (S-574-16) |
| S0B5228-P | Pacific Blue Mouse Anti-Human CD8 Antibody(S-R533) |
| S0B1527-P | FITC Mouse Anti-Human CD19 Antibody (S-1039-46) |
| S0B5931-P | PE Mouse Anti-Human CD56 Antibody (S-3499) |
| S0B5192-P | Alexa Fluor® 700 Mouse Anti-Human CD14 Antibody (S-631-50) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 583 (S0B88803) to perform live/dead discrimination and BV605 CD45 Antibody for leukocyte discrimination.
Figure 11. S0N0006 Human T+B+NK+Monocyte Panel Kit
validation gating plots
2.6 T+ B+ NK+ Monocyte Panel (S0N0007)
This six-color panel is designed for detecting T cells, B cells, NK cells, NKT cells, and monocytes in human peripheral blood. The panel is compatible with most three-laser flow cytometers. It acts as a core backbone panel — users may add fluorophore-conjugated antibodies to detect any additional markers of interest as needed.
Table 8: S0N0007 Human T+B+NK+Monocyte Panel Kit Components
| Cat. No. | Component Name |
|---|---|
| S0N0007 | Human T+ B+ NK+ Monocyte Panel Kit |
| S0B5572-P | APC-Cy7 Mouse Anti-Human CD3 Antibody (HIT3a) |
| S0B8234-P | PerCP-Cy5.5 Mouse Anti-Human CD8 Antibody (S-R533) |
| S0B1537-P | FITC Mouse Anti-Human CD4 Antibody (S-574-16) |
| S0B5672-P | PE Mouse Anti-Human CD19 Antibody (S-R503-2) |
| S0B5940-P | PE-Cy7 Mouse Anti-Human CD16 Antibody (3G8) |
| S0B5192-P | Alexa Fluor® 700 Mouse Anti-Human CD14 Antibody (S-631-50) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 452 (S0B88801) to perform live/dead discrimination and BV605 CD45 Antibody for leukocyte discrimination.
Figure 12. S0N0007 Human T+B+NK+Monocyte Panel Kit
validation gating plots
2.7 Dendritic Cell (DC) Panel
DCs are powerful antigen-presenting cells originating from bone marrow stem cells. They have unique tree-like extensions; immature DCs stay in skin and mucosal barriers to catch foreign antigens. After taking up antigens and maturing, DCs move to lymph nodes, present antigens with MHC molecules and activate naive T cells, connecting innate and adaptive immunity. DCs have two main subtypes: myeloid and plasmacytoid DCs with different roles. They trigger immune responses against germs and tumors while keeping immune balance. DCs are heavily studied to create new immunotherapy treatments.
Figure 13. Dendritic cell lineage differentiation schematic
Table 9: S0N0008 Mouse DC Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0008 | Mouse DC Panel Kit |
| S0B5085-P | APC Armenian Hamster Anti-Mouse CD11c Antibody (N418) |
| S0B1661-P | FITC Rat Anti-Mouse I-A/I-E Antibody (M5/114.15.2) |
| S0B5160-P | PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 583 (S0B88803) to perform live/dead discrimination and 1 μg Pacific Blue Rat Anti-Mouse CD45 Antibody (30-F11) (S0B1655) for leukocyte discrimination.
Figure 14. S0N0008 Mouse DC Panel Kit
validation gating plots
Table 10: S0N0011 Human DC Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0011 | Human DC Panel Kit |
| S0B5173-P | PerCP-Cy5.5 Mouse Anti-Human HLA-DR Antibody (L243) |
| S0B8092-P | PE Mouse Anti-Human CD11c Antibody (S-R424) |
| S0B1658-P | APC Rabbit Anti-Human CD86 Antibody (S-990-1) |
| S0B1552-P | PE-Cy7 Rabbit Anti-Human CD80 Antibody (S-288-177) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 452 (S0B88801) to perform live/dead discrimination.
Figure 15. S0N0011 Human DC Panel Kit
validation gating plots
2.8 Tissue-Resident Memory T (TRM) Cell Panel
TRM cells are stationary memory T cells, distinct from circulating Tcm and Tem subsets. They reside long-term in skin, lung, gut and mucosal tissues; markers CD69 and CD103 prevent their tissue exit. Re-encountered antigens prompt TRM cells to secrete IFN-γ, TNF and cytotoxic factors to locally clear pathogens and tumor cells rapidly. Local tissue niches sustain their long survival without persistent antigen exposure. TRM cells deliver frontline protection against infection and cancer, yet overactivation induces autoimmune inflammation. They are intensively studied for vaccine development and targeted cancer immunotherapy.
Figure 16. TRM cell differentiation and tissue residency regulatory pathway schematic
Table 11: S0N0009 Human Tissue-Resident T Cells Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0009 | Human Tissue-Resident T Cells Kit |
| S0B5572-P | APC-Cy7 Mouse Anti-Human CD3 Antibody (HIT3a) |
| S0B8156-P | Pacific Blue Mouse Anti-Human CD8 Antibody (S-3203) |
| S0B1537-P | FITC Mouse Anti-Human CD4 Antibody (S-574-16) |
| S0B8638-P | PE Mouse Anti-Human CD69 Antibody (FN50) |
| S0B5942-P | PerCP-Cy5.5 Mouse Anti-Human CD103 Antibody (Ber-ACT8) |
| S0B5140-P | APC Mouse Anti-Human CD62L Antibody (S-937-77) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 545 (S0B88802) to perform live/dead discrimination and AF700 CD45 Antibody for leukocyte discrimination.
Figure 17. S0N0009 Human Tissue-Resident T Cells Kit
validation gating plots
2.9 TE/TN/TCM/TEM Panel
This six-color panel is designed for characterizing T-cell subpopulations, including naïve T cells, central memory T cells, effector memory T cells, and short-lived effector T cells in mouse tissue cells. The panel is compatible with most three-laser flow cytometers. It acts as a core backbone panel — users may add fluorophore-conjugated antibodies to detect any additional markers of interest as needed.
Table 12: S0N0012 Mouse TE/TN/TCM/TEM Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0012 | Mouse TE/TN/TCM/TEM Panel Kit |
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) |
| S0B1500-P | FITC Rat Anti-Mouse CD3 Antibody (17A2) |
| S0B5936-P | PE Rat Anti-Mouse CD4 Antibody (GK1.5) |
| S0B5086-P | APC Rat Anti-Mouse CD8α Antibody (53-6.7) |
| S0B5837-P | PE-Cy7 Rat Anti-Mouse CD44 Antibody (S-923-31) |
| S0B8222-P | Pacific Blue Rat Anti-Mouse CD62L Antibody (MEL-14) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 545 (S0B88802) to perform live/dead discrimination.
Figure 18. S0N0012 Mouse TE/TN/TCM/TEM Panel Kit
multi-sample validation gating plots
2.10 Mixed Lymphocyte Reaction (MLR) Panel
MLR is a classic lab immune test discovered in 1963. It mixes lymphocytes from genetically different donors to measure T cell responses to unmatched HLA proteins on antigen-presenting cells. There are two primary MLR types. In two-way MLR, both cell populations multiply. In one-way MLR, stimulator cells are treated with radiation or mitomycin C to stop their growth. After recognizing foreign MHC antigens, reactive T cells multiply and release cytokines such as IFN-γ. This process imitates the immune reaction that causes organ transplant rejection. Researchers commonly use MLR to judge transplant matching, find immune-regulating drugs, and check how well cancer immunotherapies work.
Table 13: S0N0010 Human MLR Panel Kit Components
| Cat. No. | Product Name |
|---|---|
| S0N0010 | Human MLR Panel Kit |
| S0B5572-P | APC-Cy7 Mouse Anti-Human CD3 Antibody (HIT3a) |
| S0B5173-P | PerCP-Cy5.5 Mouse Anti-Human HLA-DR Antibody (L243) |
| S0B8092-P | PE Mouse Anti-Human CD11c Antibody (S-R424) |
| S0B1658-P | APC Rabbit Anti-Human CD86 Antibody (S-990-1) |
| S0B1552-P | PE-Cy7 Rabbit Anti-Human CD80 Antibody (S-288-177) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 452 (S0B88801) to perform live/dead discrimination and AF700 CD45 Antibody for leukocyte discrimination.
Figure 19. S0N0010 Human MLR Panel Kit
validation gating plots
2.11 Myeloid Panel
This four-color panel is designed for detecting myeloid cell subsets, including monocytes, resident macrophages, granulocytes, and conventional dendritic cells in mouse tissue cells. The panel is compatible with most two-laser flow cytometers. It acts as a core backbone panel — users may add fluorophore-conjugated antibodies to detect any additional markers of interest as needed.
Recommended co-stain: for live/dead assay
Table 14: S0N0013 Mouse Myeloid Panel Kit Components
| Cat. No. | Components |
|---|---|
| S0N0013 | Mouse Myeloid Panel Kit |
| S0B1808-P | FITC Rat Anti-Mouse/Human CD11b Antibody (M1/70) |
| S0B8017-P | PE Rat Anti-Mouse Ly-6C Antibody (HK1.4) |
| S0B5085-P | APC Armenian Hamster Anti-Mouse CD11c Antibody (N418) |
| S0B5040-P | Percp-Cy5.5 Rat Anti-Mouse I-A/I-E Antibody (M5/114.15.2) |
Notes
- This kit is recommended for co-staining with Fixable Viability Dye 545 (S0B88802) to perform live/dead discrimination and 0.25 μg Pacific Blue Rat Anti-Mouse CD45 Antibody (30-F11) (S0B1655) for leukocyte discrimination.
Figure 21. S0N0013 Mouse Myeloid Panel Kit
multi-sample validation gating plots
