CD34 Recombinant Antibody: Core Biomarker Reagent for Hematopoietic Stem Cell and Vascular Endothelial Basic Research
Molecular Profile and Biological Functions of CD34 in Stem Cell and Angiogenesis Research
CD34 represents a heavily glycosylated type I transmembrane mucin protein with a molecular mass ranging from 110 kDa to 120 kDa in human cell models.
This surface marker distributes on hematopoietic stem cells, multipotent progenitors, bone marrow stromal cells and microvascular endothelial cells within primary tissue samples.
CD34 mediates intercellular adhesion, stem cell homing and pluripotency maintenance while regulating embryonic hematopoiesis, angiogenesis and inflammatory signaling cascades.
Antibodies targeting CD34 enable specific labeling of target cell populations and serve as standard laboratory tools for stem cell and vascular developmental research pipelines.
Core Working Mechanisms of CD34 Antibody Across Multiple Standard Immunological Assay Platforms
CD34 antibodies rely on antigen-antibody specific binding to capture membrane-localized CD34 protein and generate detectable signals for quantitative or qualitative analysis.
Immunohistochemical protocols pair primary CD34 antibodies with enzyme-conjugated secondary antibodies to produce brown precipitates outlining microvascular endothelial structures on tissue slices.
Fluorophore-labeled CD34 antibodies applied in flow cytometry quantify the relative proportion of hematopoietic stem and endothelial progenitor cell populations in single-cell suspensions.
Dual immunofluorescence staining combines CD34 antibodies with endothelial co-markers such as CD31 to visualize intact tubular vascular networks in three-dimensional culture organoids.
Western blot workflows deploy CD34 antibodies to measure total CD34 protein abundance within cell lysates harvested from differentiated stem cell or endothelial culture systems.
Research Applications of CD34 Antibodies in Hematopoietic Stem Cell Developmental Studies
CD34 acts as a canonical surface marker to identify uncommitted hematopoietic stem cells and multipotent progenitor populations in primary bone marrow and peripheral blood samples.
Flow cytometry panels integrate CD34 with CD45, CD38 and CD133 to isolate pure stem cell subsets for downstream single-cell transcriptomic lineage tracing experiments.
Researchers use CD34 antibody labeling to quantify stem cell abundance within graft samples and correlate cell ratios with hematopoietic reconstitution efficiency in in vivo animal models.
Genetic editing and CAR cell culture research rely on CD34 detection to assess transduction efficiency and residual undifferentiated progenitor cell purity in lab-manufactured cell products.
Utilization of CD34 Antibodies for In Vitro and In Vivo Angiogenesis Mechanism Exploration
Unlike CD31 endothelial markers, CD34 labels both mature vascular endothelium and immature nascent microvessels for comprehensive angiogenesis phenotype characterization.
Immunohistochemical CD34 staining on fixed tumor tissue sections enables quantitative microvessel density counting for basic tumor microenvironment mechanistic research.
Preclinical animal injury and ischemia models utilize CD34 antibodies to trace de novo vascular formation within damaged, inflamed or regenerative tissue compartments.
Matrigel tube formation assays with human umbilical vein endothelial cells adopt CD34 immunofluorescence to visualize complete luminal tubular architectures under confocal microscopy.
Species-customized CD34 antibody variants support cross-species comparative endothelial research by selectively recognizing CD34 epitopes from distinct mammalian laboratory models.
Standard CD34 Antibody Protocols for Multicolor Flow Cytometry Stem Cell Quantification Assays
Flow cytometry serves as the primary quantitative platform to enumerate CD34-positive hematopoietic progenitor cells from heterogeneous primary cell mixtures.
The validated ISHAGE staining scheme combines CD34, CD45 and live/dead discriminators to calculate absolute CD34+ cell counts from bone marrow and peripheral blood specimens.
Multicolor immunophenotyping panels pair CD34 with CD117, CD33 and CD13 to distinguish myeloid and lymphoid progenitor populations in hematopoietic developmental research.
Quality control workflows for lab-generated cell culture products measure residual CD34+ progenitor cell fractions to monitor cell differentiation and product homogeneity metrics.
Fluorophore-conjugated CD34 antibody formulations require optimized spectral compensation and fluorochrome pairing to resolve dim CD34 surface signals without background interference.
Critical Experimental Controls and Optimization Tips for Consistent CD34 Antibody Staining Results
Antigen retrieval parameters require careful adjustment for formalin-fixed paraffin-embedded tissue slices sensitive to over-fixation-induced CD34 epitope degradation.
High-temperature EDTA buffer antigen retrieval protocols preserve intact CD34 epitopes and deliver uniform membrane staining across fixed tissue section samples.
Validated positive control tissues including tonsil, placental and bone marrow biopsies must run alongside unknown samples to confirm functional antibody binding activity.
Uniform scoring guidelines govern microvessel density quantification by counting CD34-positive vascular structures within pre-defined high-density tissue hot spot regions.
Distinct antibody clone variants produce variable staining intensity; research laboratories commonly adopt the validated QBEnd10 clone for consistent comparative data output.
Separate validation experiments are required to verify CD34 antibody performance on frozen tissue blocks and paraffin-embedded histological slides independently.
Batch testing monitors antibody specificity, signal sensitivity and inter-lot consistency to support long-term continuous stem cell and angiogenesis research projects.
ANT BIO PTE. LTD. CD34 Recombinant Rabbit mAb (SDT-051-68) S0B2029 Product Specification Table
| Core Product Advantage | Detailed Technical Parameter Description |
|---|---|
| High target specificity and low non-specific background | Full validation across IF, IHC and flow cytometry confirms exclusive human CD34 recognition with negligible cross-reactivity against CD31, vWF and VE-cadherin; optimized antibody titration delivers crisp membrane staining within 3D organoid vascular tissues |
| Stable performance via recombinant monoclonal production | Recombinant expression technology locks defined antibody sequences to eliminate lot-to-lot variation, removing genetic drift risks seen in traditional hybridoma-derived antibody preparations for long-term repeated experimental trials |
| Gold-standard marker for organoid vasculogenesis research | CD34 labeling tracks de novo endothelial differentiation and evaluates structural integrity of vascular networks inside stem cell-derived organoid culture systems for developmental biology research |
| Multi-assay platform compatibility | Rigorous functional testing supports confocal immunofluorescence, paraffin/frozen IHC, flow cytometry and Western blot to satisfy diverse analytical workflows within organoid and vascular research laboratories |
| Product Base Information | S0B2029 CD34 Recombinant Rabbit mAb (SDT-051-68), unconjugated rabbit-origin antibody, 500 μL volume, in-stock laboratory research antibody reagent |
Full Matching CD34 Series Research Products from ANT BIO PTE. LTD.
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S0B2029 CD34 Recombinant Rabbit mAb (SDT-051-68) Unconjugated, 500 μL
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Fluorophore conjugated CD34 mAb variants (AF488, PE, APC) for multi-color flow cytometry immunophenotyping
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Recombinant Human CD34 Protein S0P1147 for antibody specificity verification and blocking control experiments
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Secondary antibody matching kits for IHC, IF and Western blot detection of rabbit primary antibodies
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Complete IHC staining buffer kits including antigen retrieval buffer, blocking buffer and DAB chromogenic substrate
Custom Antibody Development Service Workflow from ANT BIO PTE. LTD. for Specialized Biomarker Research
ANT BIO PTE. LTD. operates a complete rabbit immunization and single B cell sorting platform for custom monoclonal antibody generation targeting cell surface biomarker proteins.
The integrated service pipeline covers antigen structural design, immunization regimen tuning, high-throughput single B cell isolation and antibody heavy/light chain gene cloning.
Recombinant antibody expression and multi-assay functional validation confirm stable epitope binding performance for custom vascular and stem cell biomarker detection workflows.
Custom unconjugated or fluorophore-tagged antibody clones can be engineered to match unique experimental requirements for cross-species or multiplex immunostaining research designs.
Summary of CD34 Recombinant Antibody Value for Modern Stem Cell and Vascular Basic Research
CD34-specific monoclonal antibodies act as indispensable core reagents for characterizing hematopoietic stem cell populations and tracking endothelial angiogenesis in diverse lab research models.
The S0B2029 recombinant rabbit anti-CD34 mAb (SDT-051-68) delivers consistent low-background staining across histological, flow cytometry and protein blotting analytical platforms.
Recombinant antibody manufacturing eliminates lot-to-lot performance fluctuations to support longitudinal organoid vasculogenesis tracing and multi-group comparative stem cell differentiation studies.
Broad compatibility with frozen and paraffin tissue slices, suspension cell flow analysis and three-dimensional organoid imaging expands experimental flexibility for developmental biology research teams.
Research groups focusing on hematopoiesis and microvascular development may contact ANT BIO PTE. LTD. to obtain full product datasheets and laboratory sample trial arrangements.
ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs
At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.
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