Anti-CD146 Antibodies for Mechanistic Research of Lipid Metabolism and Energy Homeostasis in Adipose Biology

Tissue-Specific CD146 Expression Profiles Linked to Adipose Tissue Remodeling

CD146 (also annotated MCAM) is an immunoglobulin superfamily cell adhesion molecule with restricted expression across most mature adult somatic tissues.
Its transcript and protein abundance rise sharply within white and brown adipose depots, establishing adipose-specific functional specialization.
Quantitative protein detection from obese model adipose lysates reveals positive correlation between CD146 levels and adiposity severity metrics.
Consistent CD146 upregulation is validated across high-fat diet, aging-associated and genetically modified murine obesity culture models.
This conserved expression signature across distinct obesity experimental systems positions CD146 as a core mediator of adipose tissue dysfunction.
High-specificity anti-CD146 antibody reagents are required to quantify expression gradients for comparative metabolic mechanism research.

CD146 Signaling Governs White Adipocyte Differentiation Cascades

CD146 acts as the functional cell-surface receptor for secreted ANGPTL2 ligands during preadipocyte commitment and terminal differentiation.
Ligand-receptor binding triggers downstream CREB transcriptional signaling to drive adipogenic gene expression programs in progenitor populations.
Adipocyte-specific CD146 gene ablation in murine culture models suppresses white fat mass expansion and restricts lipid droplet formation.
Genetic knockout cell lines display elevated insulin response capacity alongside reduced pro-inflammatory cytokine secretion profiles.
The ANGPTL2-CD146-CREB axis represents a defined upstream cascade governing early adipogenic lineage commitment.
Targeted antibody neutralization assays recapitulate knockout phenotypes to validate CD146’s central adipogenic regulatory function.

Dual CD146-Mediated Controls Over Lipid Turnover in Mature White Adipocytes

Activated CD146 signaling simultaneously amplifies lipogenic gene transcription and suppresses fatty acid oxidation pathways in mature adipocytes.
Sustained ligand engagement boosts intracellular neutral lipid storage capacity by upregulating acetyl-CoA carboxylase and FASN protein levels.
Parallel inhibitory signals downregulate carnitine shuttle proteins required for mitochondrial lipid catabolism and energy liberation.
CD146 also modulates chemokine and pro-inflammatory cytokine release to construct low-grade inflammatory adipose microenvironments.
Combined lipogenic and anti-lipolytic effects drive progressive adipocyte hypertrophy and adipose tissue expansion in culture.
Multiplex antibody staining pairs CD146 with lipid metabolism markers to map single-cell metabolic heterogeneity.

Galectin-1 Driven CD146 Signaling Represses Brown Adipocyte Thermogenic Activity

Within brown adipose cell populations, CD146 engages distinct galectin-1 ligands to execute regulatory signaling independent of ANGPTL2.
This alternate receptor-ligand complex suppresses uncoupling protein 1 (UCP1) transcription and mitochondrial thermogenic programming.
Dampened UCP abundance reduces cellular uncoupled respiration and total energy expenditure within brown adipocyte culture systems.
The divergent ligand-dependent outputs in white versus brown fat illustrate CD146’s context-dependent regulatory duality.
Anti-CD146 blocking antibodies disrupt galectin-1 binding to partially restore UCP1 expression and thermogenic capacity.
Dual-color immunofluorescence distinguishes CD146 surface abundance between white and brown primary adipocytes.

Preclinical Anti-CD146 Antibody Intervention for Adipose Dysfunction Research

Neutralizing anti-CD146 monoclonal antibodies disrupt endogenous ligand binding to reverse core obesity-associated phenotypes in vitro models.
Antibody treatment limits white preadipocyte differentiation and inhibits excessive neutral lipid accumulation in mature fat cell cultures.
Simultaneous de-repression of UCP1 boosts brown adipocyte energy dissipation capacity under standardized culture conditions.
Antibody-treated co-culture systems display reduced hepatic steatosis marker expression and improved insulin signaling transduction.
Suppressed adipose inflammatory cytokine secretion alleviates paracrine inflammatory cross-talk to neighboring stromal cells.
Dual regulatory effects on white and brown adipose render anti-CD146 reagents multi-functional tools for metabolic disorder mechanism screening.

Research Application Workflows for ANT BIO PTE. LTD. Anti-CD146 Antibody Panel

Murine-specific Rat Anti-Mouse CD146 (Clone S-R606) supports flow cytometry, IHC and immunofluorescence across adipose tissue specimens.
Human-target Mouse Anti-Human CD146 (Clone S-3679) quantifies CD146 abundance in human adipocyte and endothelial cell culture lines.
FIT, Biotin and Alexa Fluor conjugated variants enable multiplex co-staining with lipid and thermogenic protein markers.
Unconjugated antibody formats are compatible with immunoprecipitation to map CD146-ligand protein interaction complexes.
Tissue microarray IHC compares CD146 expression between lean and obese murine adipose depot samples.
High-throughput flow cytometry sorts CD146-positive stromal vascular subpopulations for adipogenic differentiation assays.

Core Fundamental Research Use Cases of CD146 Antibody Reagents

Adipogenic differentiation tracking uses flow cytometry to quantify CD146 surface shifts during preadipocyte maturation.
White/brown fat comparative IHC staining visualizes differential CD146 and UCP1 co-expression patterns in tissue sections.
Lipid metabolism compound screening monitors CD146 protein shifts following small molecule intervention treatments.
Stromal vascular fraction immunophenotyping isolates CD146+ mesenchymal progenitor cell subsets for differentiation studies.
Tumor angiogenesis assays detect endothelial CD146 expression to evaluate microvessel density in xenograft tissue samples.
Autoimmunity research identifies CD146-positive T lymphocyte subsets for inflammatory cytokine secretion profiling.

ANT BIO PTE. LTD. CD146 Antibody Product Portfolio

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