Absin TUNEL Cell Apoptosis Detection Kit (FITC): High-Sensitivity Solution for In Situ Apoptosis Analysis
During apoptosis, activated endonucleases in cells cleave genomic DNA between nucleosomes, resulting in DNA fragments of approximately 180-200 bp in length. In contrast, normal or proliferating cells rarely undergo such DNA fragmentation. The TUNEL Cell Apoptosis Detection Kit (FITC) utilizes Terminal Deoxynucleotidyl Transferase (TdT) to label FITC-conjugated dUTP onto the 3'-OH ends of the fragmented DNA.
FITC (Fluorescein Isothiocyanate) is excited at a wavelength of 490-495 nm and emits fluorescence at 520-530 nm, presenting a yellow-green fluorescence signal. By detecting this specific fluorescence signal through fluorescence microscopy or flow cytometry, researchers can accurately identify and localize apoptotic cells in samples.
The Absin TUNEL Cell Apoptosis Detection Kit (FITC) exhibits excellent versatility and is compatible with multiple common sample types in medical research, including:
• Paraffin-embedded sections
• Frozen sections
• Adherent cells
• Suspension cells
This broad applicability enables the kit to be widely used in various research fields such as neuroscience, cardiology, orthopedics, and oncology, facilitating in situ apoptosis detection in different tissue and cell models.
Compared with traditional apoptosis detection methods, the Absin TUNEL Cell Apoptosis Detection Kit (FITC) has obvious advantages, making it a preferred choice for researchers:
• High sensitivity: The kit can efficiently label the 3'-OH ends of fragmented DNA in apoptotic cells, enabling the detection of subtle apoptosis signals that are easily missed by other methods, ensuring the accuracy and comprehensiveness of experimental results.
• Rapid and simple operation: With a one-step reaction design, the kit simplifies the experimental process, reduces operational errors, and shortens the experimental cycle. Researchers can complete the labeling reaction quickly without complex pre-treatment steps, improving experimental efficiency.
• Accurate in situ localization: By specifically labeling apoptotic cells in situ, the kit allows researchers to observe the spatial distribution of apoptotic cells in tissues or cell populations directly, providing intuitive experimental evidence for studying the role of apoptosis in pathological processes.
4. Application Scenarios in Basic Medical Research
Given its high sensitivity and broad sample compatibility, the kit is widely applied in key areas of basic medical research related to apoptosis, such as:
• Brain tissue injury research: Detecting apoptotic nerve cells in brain tissue after injury to explore the mechanism of neural cell death and evaluate the therapeutic effect of neuroprotective drugs.
• Myocardial ischemia-reperfusion injury research: Analyzing the degree of cardiomyocyte apoptosis during ischemia-reperfusion to clarify the pathological mechanism of myocardial injury and screen potential protective agents.
• Osteoporosis research: Investigating the apoptosis of osteoblasts or osteoclasts to reveal the imbalance of bone metabolism in osteoporosis and provide a basis for the development of anti-osteoporosis drugs.
• Tumor research: Detecting apoptotic cells in tumor tissues or tumor cell lines to evaluate the effect of anti-tumor drugs on inducing tumor cell apoptosis.
5. Product Ordering Information
|
Catalog No. |
Product Name |
Key Features |
|
abs50021 |
TUNEL Cell Apoptosis Detection Kit (FITC) |
High sensitivity, one-step reaction, compatible with multiple sample types |
6. Recommended Related Products
To provide a one-stop solution for apoptosis research, Absin also offers a series of related products that can be used in conjunction with the TUNEL Cell Apoptosis Detection Kit (FITC), including:
• Fluorescence microscopy imaging reagents
• Flow cytometry staining buffers
• Apoptosis-related antibodies (e.g., Caspase family antibodies)
• Other apoptosis detection kits (e.g., Annexin V-FITC/PI Apoptosis Detection Kit, Catalog No.: abs50001)
AN BIO PTE. LTD. is committed to providing high-quality, reliable biological research reagents and comprehensive solutions for global scientific researchers. With its professional sub-brand Absin, we focus on the research and development and production of products related to cell biology, immunology, and molecular biology. Our products are widely used in academic research, drug development, and clinical diagnosis, winning the trust and recognition of numerous researchers.
We adhere to the core values of "quality first, customer-oriented", continuously optimize product performance and service quality, and strive to become a trusted partner in the field of life sciences, contributing to the progress of global biomedical research.
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