WB result of ZMYND8 Recombinant Rabbit mAb
Primary antibody: ZMYND8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Hep G2 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 132 kDa
Observed MW: 132 kDa
ZMYND8 Recombinant Rabbit mAb (S-5407)
ZMYND8 Recombinant Rabbit mAb (S-5407)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ZMYND8 |
| Synonyms | MYND-type zinc finger-containing chromatin reader ZMYND8; Cutaneous T-cell lymphoma-associated antigen se14-3 (CTCL-associated antigen se14-3); Protein kinase C-binding protein 1; Rack7; Transcription coregulator ZMYND8; Zinc finger MYND domain-containing protein 8; KIAA1125; PRKCBP1 |
| Location | Cytoplasm, Nucleus |
| Accession | Q9ULU4 |
| Clone Number | S-5407 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HepG2, Caco-2, HEK-293, Jurkat, HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
ZMYND8 is a multi-domain transcriptional regulatory protein encoded by the ZMYND8 gene, playing critical roles in DNA damage response, transcriptional regulation, and chromatin remodeling. This protein contains a PHD zinc finger domain, a MYND domain, and a bromodomain, which enable it to recognize histone post-translational modifications (such as H3K4me0 and acetylated lysines) and sense cellular redox status. ZMYND8 recognizes distinct substrates through its different domains: its PHD zinc finger recognizes the "lysine 4 unmethylated" state on the histone H3 tail, the MYND domain binds to proteins such as the transcription factor BCL6, and the bromodomain binds to acetylated lysines. Functionally, ZMYND8 serves as a critical factor in DNA double-strand break repair—following DNA damage, it is phosphorylated by ATM/ATR kinases, recruiting the NuRD chromatin remodeling complex to damage sites to promote homologous recombination repair. Additionally, it exerts antioxidant protective effects by inhibiting the aryl hydrocarbon receptor (AhR) signaling pathway to reduce reactive oxygen species accumulation. Furthermore, ZMYND8 is closely associated with the development and progression of various cancers, including breast cancer, prostate cancer, and melanoma, influencing tumor proliferation and chemoresistance through regulation of oncogene expression or DNA repair pathways. Notably, ZMYND8 possesses both activating and repressive functions in regulating gene expression—for example, it can synergize through its PHD zinc finger and MYND domains to interact with histone deacetylase complexes and repress transcription of specific genes, while also promoting expression of other genes by recruiting histone acetyltransferases. This multifaceted nature makes ZMYND8 a potential target of great interest in the fields of tumor therapy, antioxidant defense, and tissue development.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-ZMYND8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human brain. Anti-ZMYND8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-ZMYND8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-ZMYND8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ZMYND8 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
