YY1 Recombinant Rabbit mAb (S-3652-44)

Catalog Number: S0B60040 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	YY1
Synonyms	Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1 (YY-1); INO80S
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P25490
Clone Number	S-3652-44
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HeLa, Daudi, Jurkat, MDA-MB-231, T-47D, MCF7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:2000	Hu

Background

YY1 is a ubiquitously expressed multifunctional zinc finger transcription factor belonging to the GLI-Krüppel family, named for its dual "yin-yang" regulatory property of both activating and repressing target gene transcription. The key structural feature of this protein is its C-terminal region containing four tandem C2H2-type zinc finger domains responsible for sequence-specific recognition and binding to the core DNA motif (5'-CCGCCATN-3'), while the N-terminal histidine/proline-rich region and acidic domain mediate interactions with various transcriptional cofactors (such as p300 and HDACs) and epigenetic regulatory proteins. YY1 plays a central regulatory network role in cell biology, participating in numerous physiological processes including embryonic development, cell proliferation, differentiation, apoptosis, DNA repair, and viral replication. Its multifunctionality is reflected in the following: on one hand, it represses target gene transcription by directly occupying promoter regions or recruiting histone deacetylases; on the other hand, it activates gene expression by recruiting histone acetyltransferases or cooperating with other transcription factors (such as Myc and p53). YY1 also plays an important architectural role, mediating the formation of DNA loops that bring distant regulatory elements (enhancers and promoters) into close proximity, thereby coordinating the expression of complex gene loci. Clinical studies have shown that YY1 is overexpressed in most tumors (such as lymphoma, breast cancer, prostate cancer, and pancreatic cancer), promoting tumor proliferation, invasion, metastasis, and chemoresistance by regulating epithelial-mesenchymal transition, glycolytic metabolic reprogramming, and anti-apoptotic genes. Conversely, in certain tumors, YY1 can also function as a tumor suppressor. Additionally, YY1 gene mutations are associated with a rare X-linked recessive disorder—Gabriele-de Vries syndrome, characterized by developmental delay, intellectual disability, and congenital abnormalities. YY1 is also involved in regulating pluripotent stem cell self-renewal and somatic cell reprogramming, serving as a key factor in maintaining embryonic stem cell identity.

Picture

Western Blot

WB result of YY1 Recombinant Rabbit mAb
Primary antibody: YY1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Lane 6: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 65 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-YY1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.