WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: MCF7 whole cell lysate 20 μg
Lane 2: HeLa whole cell lysate 20 μg
Lane 3: SW480 whole cell lysate 20 μg
Lane 4: A549 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 49 kDa
This blot was developed with high sensitivity substrate
YB1 Recombinant Rabbit mAb (S-6245)
YB1 Recombinant Rabbit mAb (S-6245)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | YB1 |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P67809 |
| Clone Number | S-6245 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | MCF7, HeLa, SW480, A594, C2C12, NIH/3T3, RAW 264.7, C6, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Y-box-binding protein 1 (YB-1), encoded by YBX1, is a multifunctional nucleic-acid-binding member of the cold-shock domain family that shuttles between cytoplasm and nucleus (the latter upon stress); it acts as a transcription factor, co-activator or repressor by binding the Y-box (5′-CTGATTGG-3′) and other elements, regulates mRNA splicing, stability and translation by associating with mRNPs and modulating cap-dependent/IRES-driven initiation, participates in DNA repair and stress responses, and its overexpression and nuclear accumulation are linked to cancer proliferation, metastasis, multidrug resistance (via MDR1, EGFR, CCNA/B1, etc.) and poor prognosis, making it a diagnostic marker and therapeutic target.
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Picture
Western Blot
WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: C2C12 whole cell lysate 20 μg
Lane 2: NIH/3T3 whole cell lysate 20 μg
Lane 3: RAW 264.7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 49 kDa
This blot was developed with high sensitivity substrate
WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: C6 whole cell lysate 20 μg
Lane 2: PC-12 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 49 kDa
This blot was developed with high sensitivity substrate
WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Primary antibody incubation conditions: overnight at 4°C
Lane 1: COS-7 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 49 kDa
This blot was developed with high sensitivity substrate
