WB result of xCT Recombinant Rabbit mAb
Primary antibody: xCT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 60 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | xCT |
Synonyms | CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7 |
Immunogen | Synthetic Peptide |
Location | Cell membrane |
Accession | Q9UPY5 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | WB, IP |
Reactivity | Hu, Ms |
Positive Sample | K562, A549, HepG2 |
Purification | Protein A |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
application | dilution | species |
WB | 1:500-1:1000 | Hu,Ms |
IP | 1:20 | Hu,Ms |
Picture
Picture
Western Blot
