WSB1 Recombinant Rabbit mAb (S-3505-23)

Catalog Number: S0B60012 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	WSB1
Synonyms	WD repeat and SOCS box-containing protein 1; SWIP1
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	Q9Y6I7
Clone Number	S-3505-23
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:250	Hu

Background

WSB1 (WD repeat and SOCS box-containing protein 1) is an adaptor protein encoded by a gene on chromosome 17 in humans, functioning primarily as a substrate recognition subunit of the Cullin-RING E3 ubiquitin ligase complex (CRL). Its protein structure features two main functional modules: multiple WD40 repeats covering most of the sequence and a C-terminal SOCS box. The WD40 repeats are responsible for specifically recognizing and binding substrate proteins, while the SOCS box mediates interactions with core complex subunits such as Elongin B/C and Cullin-5, collectively catalyzing substrate ubiquitination and promoting their proteasomal degradation. Known degradation substrates of WSB1 include type 2 iodothyronine deiodinase (DIO2) and methylated RELA (p65), positioning it as a key player in regulating thyroid hormone metabolism and in terminating inflammatory responses in a timely manner through the degradation of NF-κB subunits. Furthermore, WSB1 exhibits broad tissue distribution, with higher expression levels in the spleen and lungs, and its dysfunction has been associated with various disease processes, including tumor proliferation and metastasis as well as neurological disorders.

Picture

Western Blot

WB result of WSB1 Recombinant Rabbit mAb
Primary antibody: WSB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293F transfected with empty vector whole cell lysate 20 µg
Lane 2: 293F transfected with WSB1-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 42 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-WSB1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.