WRN Recombinant Rabbit mAb (S-3543-129)

WRN (Werner syndrome ATP-dependent helicase) is a unique multifunctional nuclear protein in the RecQ helicase family, encoded by the WRN gene located on chromosome 8p12, containing 1,432 amino acids with a molecular weight of approximately 162 kDa. Its structure comprises an N-terminal 3'-5' exonuclease domain, a central helicase domain (containing ATPase and RecQ C-terminal domains), a protein-interaction HRDC domain, and a C-terminal nuclear localization signal, making it the only member of the RecQ family that possesses both helicase and exonuclease activities. WRN plays a central role in DNA replication, recombination, repair, telomere maintenance, and transcriptional regulation, capable of processing various DNA structures such as replication forks, Holliday junctions, and G-quadruplexes, and participates in the selection of double-strand break repair pathways through interactions with key proteins such as Ku70/80 and MRE11. Loss-of-function mutations in the WRN gene cause Werner syndrome (adult progeria); patients are normal at birth but rapidly develop premature aging manifestations after puberty, including cataracts, skin sclerosis, premature graying of hair, osteoporosis, and type 2 diabetes, with malignant tumors and myocardial infarction being the main causes of death and an average age at death of 46–54 years.