WB result of VSIG4 Recombinant Rabbit mAb
Primary antibody: VSIG4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
VSIG4 Recombinant Rabbit mAb (SDT-2737-61)
VSIG4 Recombinant Rabbit mAb (SDT-2737-61)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VSIG4 |
| Synonyms | V-set and immunoglobulin domain-containing protein 4; Protein Z39Ig; CRIg; Z39IG |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | Q9Y279 |
| Clone Number | SDT-2737-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
VSIG4 (V-set and immunoglobulin domain-containing 4), also known as CRIg, is a transmembrane glycoprotein of the B7-related family that is selectively expressed on resting tissue macrophages; it functions both as a potent negative regulator of T-cell activation and IL-2 production and as a complement receptor that binds C3b/iC3b-opsonized particles, thereby mediating rapid phagocytic clearance of pathogens while inhibiting the alternative complement pathway convertase to limit inflammation and maintain peripheral immune tolerance.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-VSIG4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-VSIG4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-VSIG4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-VSIG4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
