WB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrate
VPS34 Recombinant Rabbit mAb
VPS34 Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VPS34 |
| Synonyms | Phosphatidylinositol 3-kinase VPS34 |
| Location | Endosome |
| Accession | Q6FSR7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, Jurkat, HepG2, PC-3, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IP | 1:50 | Hu, Ms, Rt |
| IHC-P | 1:50-1:250 | Hu, Ms, Rt |
Background
VPS34, also known as PIK3C3, is the sole class III phosphatidylinositol 3-kinase in mammals and plays a central role in intracellular membrane trafficking, autophagy, and signal transduction. Its structure consists of an N-terminal C2 domain, a central PIK helical domain, and a C-terminal kinase catalytic domain, with the kinase domain specifically responsible for catalyzing the conversion of phosphatidylinositol (PtdIns) to phosphatidylinositol 3-phosphate (PI3P). It exerts its functions by forming distinct complexes with different regulatory subunits—such as VPS15 and Beclin 1—and their interaction partners: the complex I formed with ATG14 primarily regulates autophagosome formation, whereas the complex II formed with UVRAG mainly regulates endocytic trafficking and vesicular transport. The generated PI3P signaling molecule specifically recruits effector proteins containing FYVE or PX domains, thereby orchestrating key steps such as vesicle docking, membrane fusion, and endosomal sorting. Through these functions, VPS34 integrates nutrient sensing, cellular stress responses, and intracellular transport, making it essential for maintaining cellular homeostasis. Notably, dysregulation of VPS34 function has been linked to various pathological processes, including tumorigenesis and cardiovascular diseases.
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Western Blot
WB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrate
WB result of VPS34 Recombinant Rabbit mAb
Primary antibody: VPS34 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 102 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VPS34 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
