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Vinculin Recombinant Rabbit mAb (S-5405)

Vinculin Recombinant Rabbit mAb (S-5405)

Catalog Number: S0B60243 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Vinculin
Synonyms Metavinculin (MV); VCL
Location Cytoplasm, Cytoskeleton, Cell membrane
Accession P18206
Clone Number S-5405
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu, Ms, Rt, Mk
Positive Sample HeLa, A431, HepG2, K-562, LnCaP, NIH/3T3, RAW264.7, mouse heart, mouse colon, PC-12, C6, rat heart, COS-7
Purification Protein A
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:10000-1:200000 Hu, Ms, Rt, Mk
IHC-P 1:1000-1: 4000 Hu, Ms, Rt

Background

Vinculin is a highly conserved, ubiquitously expressed cytoskeletal adaptor protein that localizes to both cell-cell and cell-matrix adhesion sites, where it functions as a central regulator of mechanotransduction by coupling integrins to the actomyosin cytoskeleton through its head-domain binding to talin and its tail-domain binding to F-actin, PIP2 lipids, and multiple actin-organizing proteins, thereby stabilizing focal adhesions and adherens junctions in response to tension and phosphoinositide signals that trigger its transition from an auto-inhibited closed state to an active open conformation.

Picture

Western Blot

WB result of Vinculin Recombinant Rabbit mAb
Primary antibody: Vinculin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: K-562 whole cell lysate 20 µg
Lane 6: LnCap whole cell lysate 20 µg
Low expression control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa

WB result of Vinculin Recombinant Rabbit mAb
Primary antibody: Vinculin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Raw 264.7 whole cell lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Lane 4: mouse colon lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa

WB result of Vinculin Recombinant Rabbit mAb
Primary antibody: Vinculin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: Rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa

WB result of Vinculin Recombinant Rabbit mAb
Primary antibody: Vinculin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-Vinculin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.