WB result of Vinculin Recombinant Mouse mAb
Primary antibody: Vinculin Recombinant Mouse mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lowe expression control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
Vinculin Recombinant Mouse mAb (S-916-217)
Vinculin Recombinant Mouse mAb (S-916-217)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Vinculin |
| Synonyms | Metavinculin (MV); VCL |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Cell membrane |
| Accession | P18206 |
| Clone Number | S-916-217 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A431, HepG2, K562, NIH/3T3, C6, rat heart |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
Vinculin is a highly conserved, ubiquitously expressed cytoskeletal adaptor protein that localizes to both cell-cell and cell-matrix adhesion sites, where it functions as a central regulator of mechanotransduction by coupling integrins to the actomyosin cytoskeleton through its head-domain binding to talin and its tail-domain binding to F-actin, PIP2 lipids, and multiple actin-organizing proteins, thereby stabilizing focal adhesions and adherens junctions in response to tension and phosphoinositide signals that trigger its transition from an auto-inhibited closed state to an active open conformation.
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Western Blot
WB result of Vinculin Recombinant Mouse mAb
Primary antibody: Vinculin Recombinant Mouse mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
WB result of Vinculin Recombinant Mouse mAb
Primary antibody: Vinculin Recombinant Mouse mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 124 kDa
Observed MW: 124 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-Vinculin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
