ICC shows positive staining in HeLa cells. Anti- Vimentin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Vimentin Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-029-20)
Vimentin Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-029-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Vimentin |
| Synonyms | VIM |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P08670 |
| Clone Number | S-029-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Pr, Bv, Cz, Pg, Hm, Av |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | |
| IF | 1:500 |
Background
Vimentin is a structural protein that in humans is encoded by the VIM gene. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
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Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti-Vimentin(Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C.The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti-Vimentin(Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C.The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon cancer. Anti- Vimentin (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
