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VGLUT2 Recombinant Rabbit mAb (S-4399-14)

VGLUT2 Recombinant Rabbit mAb (S-4399-14)

Catalog Number: S0B60282 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen VGLUT2
Synonyms Vesicular glutamate transporter 2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6; Dnpi; Vglut2; Slc17a6
Immunogen Synthetic Peptide
Location Cell membrane, Synapse
Accession Q8BLE7
Clone Number S-4399-14
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Ms, Rt
Positive Sample mouse brain, rat brian
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Ms, Rt
IHC-P 1:1000 Ms, Rt

Background

VGLUT2 (Vesicular Glutamate Transporter 2), also known as SLC17A6 or DNPI, is a synaptic vesicle protein that mediates the sodium-dependent uptake of glutamate into presynaptic vesicles. As a specific marker for glutamatergic neurons, it plays a crucial role in excitatory neurotransmission and is considered a potential therapeutic target for neurological disorders involving excessive glutamate signaling, such as epilepsy, chronic pain, and neurodegenerative diseases.

Picture

Western Blot

WB result of VGLUT2 Recombinant Rabbit mAb
Primary antibody: VGLUT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65-70 kDa
Observed MW: 65 kDa

WB result of VGLUT2 Recombinant Rabbit mAb
Primary antibody: VGLUT2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65-70 kDa
Observed MW: 65 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse spleen. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-VGLUT2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.