IHC shows positive staining in paraffin-embedded human colon. Anti-VEGFA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
VEGFA Recombinant Rabbit mAb (S-5301)
VEGFA Recombinant Rabbit mAb (S-5301)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VEGFA |
| Synonyms | Vascular endothelial growth factor A, long form; L-VEGF; Vascular permeability factor (VPF); VEGF; VEGF-A |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P15692 |
| Clone Number | S-5301 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:100 | Hu |
| ICC | 1:1000-1:2000 | Hu |
Background
Vascular Endothelial Growth Factor A (VEGF-A) is a 45 kDa homodimeric glycoprotein and the founding member of the VEGF family, encoded on chromosome 6p21.3 and expressed as at least seven splice isoforms (e.g., VEGF-A165) that are secreted or extracellular-matrix-bound to initiate angiogenesis and vascular permeability through binding VEGFR-2 on endothelial cells and neuropilin co-receptors, triggering PI3K-Akt, PLCγ-MAPK, and Src signaling cascades that drive endothelial proliferation, migration, and tube formation, while its transcription is up-regulated by hypoxia via HIF-1α and by growth factors such as PDGF and FGF, and its dysregulation underlies tumor neovascularization, diabetic retinopathy, and inflammatory disorders, making VEGF-A a pivotal therapeutic target for anti-angiogenic cancer drugs and anti-VEGF antibodies like bevacizumab.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-VEGFA antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-VEGFA antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
