WB result of VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 152 kDa
Observed MW: 200~260 kDa
VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (S-1519-34)
VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (S-1519-34)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VEGF Receptor/FLK-1 |
| Synonyms | Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase insert domain receptor (KDR); Protein-tyrosine kinase receptor flk-1; CD309; KDR |
| Immunogen | Recombinant Protein |
| Location | Cell membrane, Cell junction |
| Accession | P35968 |
| Clone Number | S-1519-34 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Positive Sample | HUVEC |
| Predicted Reactivity | P. paniscus, P. troglodytes |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
Background
VEGF Receptor 2 (VEGFR2), also known as kinase insert domain receptor (KDR) in humans, is a crucial tyrosine kinase receptor involved in angiogenesis, the formation of new blood vessels. It plays a significant role in various physiological and pathological processes, including development, wound healing, and tumor growth. VEGFR2 is activated upon binding to VEGF (Vascular Endothelial Growth Factor), leading to a series of downstream signaling events that promote endothelial cell proliferation, migration, and survival. This receptor is composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. Upon VEGF binding, VEGFR2 undergoes dimerization and autophosphorylation, which activates various signaling pathways such as PI3K/Akt, MAPK/ERK, and others.
Picture
Picture
Western Blot
IP
VEGF Receptor 2/FLK-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VEGF Receptor 2/FLK-1 in 0.4 mg HUVEC whole cell lysate.
Western blot was performed on the immunoprecipitate using VEGF Receptor 2/FLK-1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HUVEC whole cell lysate 20 µg (Input)
Lane 2: VEGF Receptor 2/FLK-1 Rabbit mAb IP in HUVEC whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HUVEC whole cell lysate
Predicted MW: 152 kDa
Observed MW: 200~260 kDa
This blot was developed with high sensitivity substrate
