WB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 250, 280 kDa
This blot was developed with high sensitivity substrate
VEGF Receptor 2 Recombinant Rabbit mAb (S-3912-20)
VEGF Receptor 2 Recombinant Rabbit mAb (S-3912-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VEGF Receptor 2 |
| Synonyms | Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase insert domain receptor (KDR); Protein-tyrosine kinase receptor flk-1; CD309; FLK1; VEGFR2; KDR |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Endoplasmic reticulum |
| Accession | P35968 |
| Clone Number | S-3912-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HUVEC, mouse brain, mouse heart, rat heart |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
VEGF Receptor 2 (VEGFR-2), also known as KDR in humans and Flk-1 in mice, is a crucial receptor tyrosine kinase that plays a central role in angiogenesis, the formation of new blood vessels. This receptor has high affinity for VEGF-A and mediates most of the endothelial growth, migration, and survival signals induced by VEGF-A. Structurally, VEGFR-2 is a glycosylated transmembrane protein with a molecular weight of about 230 kDa, consisting of an extracellular domain (ECD) for ligand binding, a transmembrane domain (TMD), a juxtamembrane domain (JMD), a tyrosine kinase domain (TKD), and a C-terminal domain (CTD) for signaling. Upon binding to VEGF-A, VEGFR-2 undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as the PLCγ-PKC-MAPK pathway, which promotes endothelial cell proliferation, migration, and vascular permeability. VEGFR-2 is widely expressed in vascular endothelial cells and is also found in some tumor cells, making it a key target for both pro-angiogenic therapies in conditions like ischemic diseases and anti-angiogenic therapies in cancer.
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Western Blot
WB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 280 kDa
This blot was developed with high sensitivity substrate
WB result of VEGF Receptor 2 Recombinant Rabbit mAb
Primary antibody: VEGF Receptor 2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 152 kDa
Observed MW: 210, 280 kDa
This blot was developed with high sensitivity substrate
