WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 130 kDa
VE-Cadherin Recombinant Rabbit mAb (S-1291-239)
VE-Cadherin Recombinant Rabbit mAb (S-1291-239)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD144 |
| Synonyms | Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P33151 |
| Clone Number | S-1291-239 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse heart, rat heart |
| Predicted Reactivity | Pg, Ck |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:2000 | Ms, Rt |
Background
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation.
Picture
Picture
Western Blot
WB result of VE-Cadherin Recombinant Rabbit mAb
Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 130 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse lung. Anti- VE-Cadherin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- VE-Cadherin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
