WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
VDAC1 + VDAC2 + VDAC3 Rabbit Polyclonal Antibody
VDAC1 + VDAC2 + VDAC3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VDAC1 + VDAC2 + VDAC3 |
| Synonyms | Voltage-dependent anion-selective channel protein; hVDAC1; Non-selective voltage-gated ion channel VDAC2; VDAC-2; Non-selective voltage-gated ion channel VDAC3; VDAC-3; hVDAC3; Outer mitochondrial membrane protein porin 3 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion, Cell membrane, Endoplasmic reticulum |
| Accession | P21796、Q9MZ15、Q9Y277 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, A431, Jurkat, HEK-293, HepG2, NIH/3T3, mouse heart, PC-12, rat heart |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
VDAC1, VDAC2, and VDAC3 are three closely related ~30 kDa β-barrel porins embedded in the mitochondrial outer membrane where they form voltage-dependent anion channels that collectively serve as the primary gatekeepers for metabolite flux—allowing ATP/ADP, NADH, ion, and other small-molecule exchange between cytosol and mitochondria—while also acting as hubs for Ca²⁺ signaling, apoptotic protein binding, and interaction with hexokinase, creatine kinase, Bcl-2 family proteins, and cytoskeletal elements; despite high sequence similarity, isoforms show tissue-specific expression (VDAC1 ubiquitous, VDAC2 enriched in heart/testis and uniquely anti-apoptotic via Bax inhibition, VDAC3 prominent in testis and cilia with redox-sensitive cysteines linked to sperm motility and ROS regulation), distinct electrophysiological gating properties, and differential roles in oxidative stress, mitochondrial dynamics, and apoptosis, making the trio essential for bioenergetics, cell survival decisions, and increasingly recognized as therapeutic targets in cancer, neurodegeneration, and metabolic disorders.
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Western Blot
WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
