VDAC1 Recombinant Rabbit mAb (S-5314)

Catalog Number: S0B60130 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$70 USD

Size: 25μl 100μl 1ml

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	VDAC1
Synonyms	Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1); VDAC
Location	Cell membrane, Mitochondrion, Endoplasmic reticulum
Accession	P21796
Clone Number	S-5314
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HEK-293, HeLa, A431, Jurkat, MCF7, COLO205, HL-60, NIH/3T3, C2C12, PC-12, C6, rat brain
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:10000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

VDAC1 (Voltage-Dependent Anion Channel 1) is a key channel protein highly expressed on the outer mitochondrial membrane of eukaryotic cells and is a major member of the VDAC family. Structurally, it consists of 19 β-strands that form a transmembrane β-barrel structure, whose internal pore size can switch between "open" and "closed" states in response to changes in membrane potential, thereby regulating the transmembrane transport of various ions (such as Cl⁻ and K⁺) and metabolites (such as ATP, ADP, and pyruvate), serving as the central gateway for material exchange between mitochondria and the cytoplasm. Functionally, VDAC1 is far more than a passive channel; it dynamically interacts with a variety of ligands, including hexokinase and Bcl-2 family proteins, and is deeply involved in cellular energy metabolism, calcium homeostasis maintenance, and early regulation of apoptosis. When bound to pro-apoptotic proteins such as Bax, it can induce outer mitochondrial membrane permeabilization, releasing cytochrome c to initiate apoptosis, whereas anti-apoptotic proteins inhibit this process by occluding VDAC1. Consequently, aberrant expression or dysfunction of VDAC1 is closely associated with various diseases, including neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease, myocardial ischemia-reperfusion injury, and various cancers, making it a popular target in drug therapy and disease diagnosis research.

Picture

Western Blot

WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: COLO205 whole cell lysate 20 µg
Lane 7: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa

WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa

WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti-VDAC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.