WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HEK-293 whole cell lysate 20 µg
Lane 2: unboiled HeLa whole cell lysate 20 µg
Lane 3: unboiled A431 whole cell lysate 20 µg
Lane 4: unboiled Jurkat whole cell lysate 20 µg
Lane 5: unboiled MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
VDAC1 Recombinant Rabbit mAb (S-1877-18)
VDAC1 Recombinant Rabbit mAb (S-1877-18)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VDAC1 |
| Synonyms | Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin, Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1); VDAC |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion, Cell membrane, Endoplasmic reticulum |
| Accession | P21796 |
| Clone Number | S-1877-18 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | HEK-293, HeLa, A431, Jurkat, MCF7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
Voltage-dependent anion channel 1 (VDAC1) is a multifunctional protein located in the outer mitochondrial membrane (OMM), playing a crucial role in regulating cellular metabolism and apoptosis. VDAC1 facilitates the transport of metabolites, ions, and small molecules between the mitochondria and the cytosol, including ATP, ADP, and calcium ions, thereby controlling the metabolic cross-talk between these cellular compartments. Additionally, VDAC1 is involved in mitochondria-mediated apoptosis by forming channels that release apoptotic proteins such as cytochrome c into the cytosol. It also interacts with over 150 proteins, including anti-apoptotic proteins like Bcl-2 and hexokinase, which are often overexpressed in cancer cells, making VDAC1 a potential target for cancer therapy.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human prostate. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
