WB result of VDAC1 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: mouse kidney lysate 20 µg
Primary antibody:
Lane 1: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 32 kDa
Observed MW: 32 kDa
VDAC1 Recombinant Rabbit mAb (HRP Conjugate) (SDT-R013)
VDAC1 Recombinant Rabbit mAb (HRP Conjugate) (SDT-R013)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VDAC1 |
| Synonyms | Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM |
| Location | Cell membrane, Mitochondrion outer membrane |
| Accession | P21796 |
| Clone Number | SDT-R013 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | mouse kidney, rat kidney |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Ms, Rt |
Background
Voltage-dependent anion-selective channel 1 (VDAC-1) is a beta barrel protein that in humans is encoded by the VDAC1 gene located on chromosome 5. It forms an ion channel in the outer mitochondrial membrane (OMM) and also the outer cell membrane. In the OMM, it allows ATP to diffuse out of the mitochondria into the cytoplasm. In the cell membrane, it is involved in volume regulation. Within all eukaryotic cells, mitochondria are responsible for synthesis of ATP among other metabolite needed for cell survival. VDAC1 therefore allows for communication between the mitochondrion and the cell mediating the balance between cell metabolism and cell death. Besides metabolic permeation, VDAC1 also acts as a scaffold for proteins such as hexokinase that can in turn regulate metabolism.
Picture
Picture
Western Blot
WB result of VDAC1 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: rat kidney lysate 20 µg
Primary antibody:
Lane 1: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: VDAC1 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 32 kDa
Observed MW: 32 kDa
