WB result of VANGA2 Recombinant Rabbit mAb
Primary antibody: VANGA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDa
VANGA2 Recombinant Rabbit mAb (S-2938-231)
VANGA2 Recombinant Rabbit mAb (S-2938-231)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VANGA2 |
| Synonyms | Vang-like protein 2; Loop-tail protein 1 homolog; Strabismus 1; Van Gogh-like protein 2; KIAA1215; STB1; VANGL2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9ULK5 |
| Clone Number | S-2938-231 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Ms, Rt |
| Positive Sample | NIH/3T3, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| ICC | 1:100 | Ms |
Background
VANGL2 (Van Gogh-like 2) is a four-pass transmembrane core component of the Wnt/planar cell polarity (PCP) pathway that—by shuttling between the endoplasmic reticulum, plasma membrane and endocytic vesicles—governs embryonic convergence-extension, neural crest migration, adult tissue homeostasis and repair, while in disease contexts it drives malignant traits: in glioma it is transcriptionally up-regulated, correlates with IDH mutation and poor prognosis, and accelerates proliferation, invasion and stem-like sphere formation via positive crosstalk with Notch signaling, whereas in inflammatory disorders it unexpectedly suppresses NF-κB by acting as an autophagy adaptor that recruits the E3 ligase PDLIM2 to K63-ubiquitinate the p65 subunit, thereby enabling NDP52-mediated selective autophagic degradation of p65 and limiting pro-inflammatory cytokine output.
Picture
Picture
Western Blot
WB result of VANGA2 Recombinant Rabbit mAb
Primary antibody: VANGA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 62 kDa
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- VANGA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
