WB result of Uteroglobin Recombinant Rabbit mAb
Primary antibody: Uteroglobin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Low expression control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 9 kDa
Uteroglobin Recombinant Rabbit mAb (SDT-2736-46)
Uteroglobin Recombinant Rabbit mAb (SDT-2736-46)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Uteroglobin |
| Synonyms | Club cell phospholipid-binding protein (CCPBP); Club cells 10 kDa secretory protein (CC10); Secretoglobin family 1A member 1; Urinary protein 1 (UP-1; UP1; Urine protein 1); CC10; CCSP; UGB; SCGB1A1 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P11684 |
| Clone Number | SDT-2736-46 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu |
| Positive Sample | BxPC-3 |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
| IF | 1:200 | Hu |
Background
Uteroglobin, also known as secretoglobin 1A1 or blastokinin, is a small, 70–77 amino acid, disulfide-linked homodimeric protein that is secreted by the Clara (non-ciliated bronchiolar exocrine) cells of the pulmonary airways, the epithelial cells of the endometrium and the prostate, and is found in high concentrations in the uterine fluid of rabbits and other mammals during early pregnancy, where it functions as a potent anti-inflammatory, immunomodulatory, and anti-fibrotic agent by binding and sequestering hydrophobic ligands such as phospholipids, prostaglandins, and the lipid-derived aldehyde 4-hydroxy-2-nonenal, thereby protecting tissues from oxidative, allergic, and inflammatory damage, and has been implicated in the regulation of blastocyst implantation, angiogenesis, and tumor suppression through its ability to inhibit the transcription factor NF-κB and to prevent the activation of the NLRP3 inflammasome, making it a promising therapeutic target for respiratory, cardiovascular, renal, and neoplastic diseases.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung. Anti-Uteroglobin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Uteroglobin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Uteroglobin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-Uteroglobin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human lung. Anti-Uteroglobin antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
