USP14 Recombinant Rabbit mAb (S-4022-13)

Catalog Number: S0B6948 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	USP14
Synonyms	Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; TGT
Immunogen	Synthetic Peptide
Location	Cell membrane, Cytoplasm
Accession	P54578
Clone Number	S-4022-13
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, Molt-4, SK-OV-3, DU 145, Neuro-2a, L-929, mouse heart, C6, rat heart
Predicted Reactivity	Bv, Cz, Rb
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:800	Hu

Background

USP14 (Ubiquitin Specific Peptidase 14) is a deubiquitinating enzyme that serves as a critical regulatory subunit of the 26S proteasome, functioning primarily at the interface between the 19S regulatory particle and the 20S core particle to modulate protein degradation. By reversibly removing ubiquitin chains from substrate proteins prior to their entry into the proteolytic core, USP14 acts as a "gatekeeper" that can either rescue substrates from degradation or fine-tune the rate of proteolysis, thereby playing a pivotal role in maintaining cellular protein homeostasis and regulating key physiological processes such as DNA damage response, oxidative stress management, and immune signaling. Due to its ability to stabilize oncoproteins and influence tumor cell survival, USP14 has emerged as a significant therapeutic target in cancer research, where its inhibition is being explored to enhance the degradation of disease-causing proteins and sensitize tumors to chemotherapy, highlighting its dual potential as both a protector of cellular integrity and a driver of pathological states when dysregulated.

Picture

Western Blot

WB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa

WB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: L-929 whole cell lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa

WB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa

WB result of USP14 Recombinant Rabbit mAb
Primary antibody: USP14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-USP14 antibody was used at 1/800 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.