Urokinase Recombinant Rabbit mAb (S-5949)

Catalog Number: S0B60547 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Urokinase
Location	Secreted
Accession	P00749
Clone Number	S-5949
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Ramos, LNCaP, PC-3, MCF7, DU145, HeLa
Purification	Protein A
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:1000	Hu

Background

Urokinase-type plasminogen activator (uPA) is a 411-residue, trypsin-like serine protease that converts the abundant zymogen plasminogen into the broad-spectrum protease plasmin; by binding to its high-affinity glycolipid-anchored receptor uPAR on the plasma membrane, uPA focuses pericellular proteolysis to the cell surface, thereby driving physiological extracellular-matrix remodeling during wound healing and tissue involution as well as pathological processes such as tumor cell invasion, angiogenesis and metastasis, while its non-proteolytic, growth-factor-like amino-terminal fragment (ATF) triggers intracellular signaling cascades that promote cell migration, proliferation and survival, making the uPA/uPAR system a key therapeutic target in cancer, fibrosis and thrombosis.

Picture

Western Blot

WB result of Urokinase Recombinant Rabbit mAb
Primary antibody: Urokinase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW:50-60 kDa
This blot was developed with high sensitivity substrate

WB result of Urokinase Recombinant Rabbit mAb
Primary antibody: Urokinase Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: DU145 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50-60 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Urokinase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Urokinase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-Urokinase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.