uPAR/CD87 Recombinant Rabbit mAb (S-3960-82)

Catalog Number: S0B60419 Application: WB, IHC-P, ICC Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	uPAR/CD87
Synonyms	Urokinase plasminogen activator surface receptor; U-PAR; uPARAlternative nameMonocyte activation antigen Mo3; MO3; UPAR; PLAUR
Immunogen	Recombinant Protein
Location	Cell membrane
Accession	Q03405
Clone Number	S-3960-82
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu
Positive Sample	U-87 MG, BxPC-3, DU 145, MDA-MB-231, U-937 cells treated with TPA (200nM, 72hr)
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu
IHC-P	1:500-1:2000	Hu
ICC	1:500	Hu

Background

CD87 is a glycoprotein encoded by the PLAUR gene and anchored to the cell membrane surface via glycosylphosphatidylinositol (GPI), playing a critical role in cell migration, tissue remodeling, and signal transduction. As a high-affinity receptor for urokinase-type plasminogen activator (uPA), CD87 confines uPA-mediated plasminogen activation to the cell surface, thereby promoting localized degradation of the extracellular matrix—a function that is essential in both physiological processes such as wound healing, embryo implantation, and angiogenesis, as well as pathological processes including tumor invasion, metastasis, and inflammation. In addition, beyond serving as a platform for proteolysis, CD87 participates in the regulation of intracellular signaling pathways through interactions with integrins, urokinase-type plasminogen activator, and vitronectin, thereby influencing cell adhesion, proliferation, and survival. This protein also exists in a soluble form (suPAR), released into the blood upon proteolytic cleavage of cell-surface CD87 or disruption of its GPI anchor, and its circulating levels correlate with the severity and prognosis of various diseases. Clinically, CD87 is highly expressed in multiple malignancies (such as breast cancer, colorectal cancer, and hepatocellular carcinoma) and is closely associated with poor patient prognosis, making it a potential prognostic biomarker and therapeutic target. Furthermore, soluble CD87 (suPAR) has been established as a novel biomarker reflecting immune activation status in chronic inflammatory and infectious diseases (such as sepsis, HIV, and tuberculosis), capable of predicting disease progression and patient outcomes.

Picture

Western Blot

WB result of uPAR/CD87 Recombinant Rabbit mAb
Primary antibody: uPAR/CD87 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: BxPC-3 whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Lane 5: MDA-MB-231 whole cell lysate 20 µg
Lane 6: untreated U-937 whole cell lysate 20 µg
Lane 7: U-937 treated with 200nM TPA for 72 hours whole cell lysate 20 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 35-65 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-uPAR/CD87 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung. Anti-uPAR/CD87 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-uPAR/CD87 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-uPAR/CD87 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-uPAR/CD87 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-uPAR/CD87 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC analysis of U-937 cells treated with TPA (200nM, 72hr) (top panel) and untreated U-937 cells (below panel). Anti- uPAR/CD87 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).