UniOne® TR-FRET Human KRAS[G12C]/CYPA/BRAF Quaternary Binding Kit

The kit is based on homogeneous time-resolved fluorescence technology (TR-FRET) technology，which is used to assess the molecular-mediated interaction between the KRAS[G12C] and BRAF. This method provides a high-throughput approach to detect the small molecules capable of mediating the interaction between the KRAS[G12C] and BRAF simply and rapidly.

As shown in the figure below, the interaction between KRAS[G12C] and BRAF was detected by an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET receptor). The donor antibody is in close proximity to the receptor antibody. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the receptor antibody, resulting in specific emission of a signal at a wavelength of 665 nm from the receptor antibody. And the molecular adhesive Daraxonrasib mediates the interaction between KRAS[G12C] and CypA, which competed with BRAF. Resulting the specific emission of a signal at a wavelength of 665 nm from the receptor antibody decreased. This decrease of specific signal is proportional to the degree of interaction between Daraxonrasib, KRAS[G12C] and CypA. This homogeneous assay is simple to perform and requires no washing steps.

【Composition and Storage Conditions】

Note: Aliquot into appropriate volumes, store at the recommended temperature and avoid repeated freeze-thaw cycle.