UniOne® TR-FRET Human IL4/IL4RA Binding Kit

This kit employs Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL4 and IL4R. Thismethod enables simple and rapid high-throughput screening of inhibitors andantibody blockers.

As illustrated, the interaction between IL4 and IL4R isdetected using an Eu-labeled Anti-Tag1 antibody (TR-FRET donor) and anAcceptor-labeled IL4 protein (TR-FRET acceptor). The binding of IL4 to IL4Rbrings the donor antibody and the acceptor-labeled IL4 into close proximity.Excitation of the donor antibody triggers Fluorescence Resonance EnergyTransfer (FRET) to the acceptor antibody, resulting in a specific emissionsignal at 665 nm. The intensity of this specific signal is proportional to theextent of the IL4 and IL4R interaction. This homogeneous assay is simple toperform and requires no washing steps.