UniOne® TR-FRET Human IL3/IL3R Binding Kit

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between IL3 and IL3R. Thismethod enables simple and rapid high-throughput screening of inhibitors andblocking antibodies.

As shown, the interaction between IL3 and IL3R isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled IL3 protein (TR-FRET acceptor). The binding of IL3 to IL3Rbrings the donor and acceptor antibodies into proximity. Excitation of thedonor antibody triggers Fluorescence Resonance Energy Transfer (FRET) to theacceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL3 andIL3R. This homogeneous assay is simple to perform and requires no washingsteps.

Table 1. Kit Components and Storage Conditions

*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.