UniOne® TR-FRET Human IL2/IL2R Binding Kit

This kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL2 and IL2R. This method enables simple and rapid high-throughputscreening of inhibitors and blocking antibodies.

As shown, the interaction betweenIL2 and IL2R is detected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor)and an Acceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). The binding ofIL2 to IL2R brings the donor and acceptor antibodies into proximity. Excitationof the donor antibody triggers Fluorescence Resonance Energy Transfer (FRET) tothe acceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL2 andIL2R. This homogeneous assay is simple to perform and requires no washingsteps.

*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.